%0 Journal Article
%T Spectrophotometric Determination of Zolmitriptan in Bulk Drug and Pharmaceuticals Using Vanillin as a Reagent
%A Kudige N. Prashanth
%A Kanakapura Basavaiah
%A Madihalli S. Raghu
%J ISRN Analytical Chemistry
%D 2013
%R 10.1155/2013/790382
%X An accurate and precise spectrophotometric method is presented for the determination of zolmitriptan (ZMT) based on the formation of a red color product with vanillin in presence of concentrated H2SO4, with the chromogen being measured at 580£¿nm. The reaction proceeds quantitatively at room temperature in 10£¿min. The calibration curve is linear over the range 5.0¨C90.0£¿¦Ìg£¿mL£¿1 and described by the regression equation with a regression coefficient of 0.9994 . The calculated molar absorptivity and Sandell sensitivity values are 3.3 ¡Á 103 L£¿mol£¿1£¿cm£¿1 and 0.0872£¿¦Ìg£¿cm£¿2, respectively. The limits of detection (LOD) and quantification (LOQ) calculated as per ICH guidelines are 1.26 and 3.81£¿¦Ìg£¿mL£¿1, respectively. The within-day accuracy expressed as relative error was better than 1.78% with precision (RSD) ranging from 0.83 to 1.45%. The between-day accuracy ranged from 1.21 to 1.84% with a precision less than 1.66%. The method was successfully applied to the analysis of one brand of tablet containing zolmitriptan. The results obtained were in agreement with those obtained by published reference method. The accuracy was also checked by placebo blank and synthetic mixture analyses besides recovery study via standard addition procedure. 1. Introduction The antimigraine drug zolmitriptan (Figure 1) is a selective agonist of serotonin (5-hydroxytryptamine; 5-HT) type 1B and 1D receptors and chemically known as (4S)-4-[[3-[2-(dimethylamino) ethyl]-1H-indol-5-yl] methyl]-2-oxazolidinone. Zolmitriptan (ZMT) binds with high affinity to human 5-HT1B and 5-HT1D receptors leading to cranial blood vessel constriction. The therapeutic activity of ZMT for the treatment of migraine headache can most likely be attributed to the agonist effects at the 5HT1B/1D receptors on intracranial blood vessels (including the arteriovenous anastomoses) and sensory nerves of the trigeminal system, which result in cranial vessel constriction and inhibition of proinflammatory neuropeptide release [1]. Figure 1: Structure of ZMT. ZMT is not included in any pharmacopeia. Literature survey reveals that few analytical methods have been published for analysis of ZMT in human plasma and include high-performance liquid chromatography (HPLC) with coulometric [2], mass spectrometric detection [3¨C5], and liquid chromatography-mass spectrometry [1, 6, 7]. High-performance liquid chromatography (HPLC) with UV-detection has been widely used for the quantitative determination of ZMT in pharmaceuticals [8¨C15]. Ultraperformance liquid chromatography (UPLC) [16], liquid chromatography-mass spectrometry
%U http://www.hindawi.com/journals/isrn.analytical.chemistry/2013/790382/