%0 Journal Article
%T Whole Body Microwave Irradiation for Improved Dacarbazine Therapeutical Action in Cutaneous Melanoma Mouse Model
%A Monica Neagu
%A Carolina Constantin
%A Diana Martin
%A Lucian Albulescu
%A Nicusor Iacob
%A Daniel Ighigeanu
%J Radiology Research and Practice
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/414816
%X A cutaneous melanoma mouse model was used to test the efficacy of a new therapeutical approach that uses low doses of cytostatics in conjunction with mild whole body microwave exposure of 2.45£¿GHz in order to enhance cytostatics antitumoral effect. Materials and Methods. A microwave exposure system for C57BL/6 mouse whole body microwave irradiation was designed; groups of 40 mice (males and females) bearing experimental tumours were subjected to a combined therapy comprising low doses of dacarbazine in combination with mild whole body irradiation. Clinical parameters and serum cytokine testing using xMAP technology were performed. Results. The group that was subjected to combined therapy, microwave and cytostatic, had the best clinical evolution in terms of overall survival, tumour volume, and metastatic potential. At day 14 the untreated group had 100% mortality, while in the combined therapy group 40% of mice were surviving. Quantifying serum IL-1¦Â, IL-6, IL-10, IL-12 (p70), IFN-¦Ã, GM-CSF, TNF-¦Á, MIP-1¦Á, MCP-1, and KC during tumorigenesis and therapy found that the combined experimental therapy decreases all the inflammatory cytokines, except chemokine MCP-1 that was found increased, suggesting an increase of the anti-tumoral immune response triggered by the combined therapy. The overall metastatic process is decreased in the combined therapy group. 1. Introduction Malignant melanoma (MM) is one of the most aggressive human cancers, since a few mm thick tumors have full potential to kill the host in more than 80% of the cases [1]. Besides the surgical elimination of the primary tumor, there is no other effective cure for MM [1, 2]. MM is resistant to ionizing radiations (radiotherapies) as well as to conventional chemotherapies. The combination of ionizing radiation as well as nonionizing radiation (such as microwaves) with other therapies is reported as a promising strategy in cancer therapy [2]. Microwaves (MW) are presently used or under study for therapeutic applications in areas such as cardiology, urology, general surgery, ophthalmology, and oncology. MW is used as well for organ imaging in the clinical diagnostic of cancer [3]. In the last years there is a revival of therapeutical possibilities to use MW in oncology, in both animal in vivo models studies and in clinical trials. Low-intensity microwave radiation used in animal model inoculated with sarcoma 45 cell line has shown that in 50% of animals¡¯ tumor growth and partial regression was obtained. The treatment was efficient due to the actual destruction of tumors and accumulation of
%U http://www.hindawi.com/journals/rrp/2013/414816/