%0 Journal Article
%T Is There Any Relationship between Human Herpesvirus-8 and Multiple Myeloma?
%A Mohammad Hadi Sadeghian
%A Maryam Mohammadnia Avval
%A Hossein Ayatollahi
%A Mohammad Reza Keramati
%A Bahram Memar
%A Saeed Amel Jamedar
%A Maryam Sheikhi
%A Gohar Shaghayegh
%J Lymphoma
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/123297
%X Background. Human herpesvirus-8 (HHV-8) is associated with some human diseases including KaposiĄ¯s sarcoma and also some B-cell lymphoproliferative disorders. Few studies have highlighted the potential role of HHV-8 in the development of multiple myeloma (MM) which is known as a malignant proliferation of plasma cells derived from a single clone. Aims. The aim of this study was to find a relationship between HHV-8 and MM using polymerase chain reaction (PCR) method. Materials and Methods. This study was conducted on 30 formalin-fixed, paraffin-embedded (FFPE) bone marrow biopsies of multiple myeloma and 30 normal FFPE bone marrow biopsies. After the sample preparation, Deoxyribonucleic acid (DNA) was extracted by nonheating procedure. PCR for HHV-8 virus was carried out with commercial kit and the PCR products were visualized by gel electrophoresis. Finally, the statistical analysis was performed. Results. HHV-8 virus was not detected by PCR from FFPE blocks of multiple myeloma samples, while only one of the controls showed DNA band of the corrected molecular weights. FisherĄ¯s exact test showed that no statistical differences were found between the two groups ( ). Conclusion. Our report adds to the body of evidence that there is no association between HHV- 8 and MM against a major role of HHV-8 infection in the pathogenesis of clonal plasma cell proliferation. 1. Introduction Human herpesvirus-8 (HHV-8), also called KaposiĄ¯s sarcoma-associated herpesvirus (KSHV), is a member of the gamma herpesvirus family [1]. This virus was first identified in KaposiĄ¯s sarcoma tissues obtained from patients with acquired immunodeficiency syndrome (AIDS) by Chang et al. in 1994 [2]. It has been implicated that HHV-8 may be associated with some human diseases including primary effusion lymphoma, a rare subtype of B-cell non-Hodgkin's lymphoma (NHL) [3], multicentric Castleman disease [4], angiosarcoma [5], angiolymphoid hyperplasia with eosinophilia [6], sarcoidosis [7], squamous cell carcinoma [8], pityriasis rosea [9], and multiple myeloma (MM) [10]. Multiple myeloma or plasma cell myeloma is a hematologic malignancy caused by neoplastic plasma cells which synthesize abnormal amounts of monoclonal immunoglobulins or immunoglobulin fragments [11]. This is primarily a disease of the middle-aged and the elderly with a median age at diagnosis of about 62 years [12] that affects slightly more men than women [13]. Plasma cell myeloma is the second most common blood malignancy in USA after NHL, causing about 1% of human malignancies and 13% of hematological neoplasms [12]. It
%U http://www.hindawi.com/journals/lymph/2013/123297/