%0 Journal Article
%T Isolation and Molecular Characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in Chickens in Sudan
%A Khalda A. Khalifa
%A Egbal Sidahmed Abdelrahim
%A Magdi Badwi
%A Amal M. Mohamed
%J Journal of Veterinary Medicine
%D 2013
%R 10.1155/2013/208026
%X The current study described the isolation and molecular detection of Mycoplasma gallisepticum (Mg) and Mycoplasma synoviae from tracheal swabs of diseased birds showing signs of respiratory distress in selected commercial (layer and broiler) farms and from yolk and an open air of pens of vaccinated breeder flocks in Sudan. A number of 45£¿Mycoplasma isolates were recovered from chickens in Khartoum, Gezira, and Equatoria states in Sudan. Of these, eight Mg and three Ms isolates were identified using growth inhibition and rapid serum agglutination (RSA) tests. The conventional PCR technique was applied to amplify 140£¿bp and 720£¿bp DNA fragments for the Mg and Ms, respectively. This research confirmed vertical and horizontal transmission of Mg from breeder farms through detection of Mg in yolk of fertile eggs and an air of pens despite previous vaccination. PCR is considered a rapid, sensitive, and cheap method and it will improve the diagnosis of Mycoplasma in chickens. 1. Introduction Avian mycoplasmosis was primarily described in turkeys in 1926 and in chickens in 1936 [1]. Mycoplasma gallisepticum (Mg) infection is usually designated as chronic respiratory disease of chickens and infectious sinusitis in turkeys. It is characterized by respiratory rales, coughing, nasal discharges, and frequently by sinusitis in turkeys by synovitis. Mycoplasma synoviae (Ms) infection is usually known as infectious synovitis, an acute-to-chronic infectious disease for chickens and turkeys involving primarily the synovial membranes of joints and tendons sheaths. However, during recent years, Ms has less frequently been associated with synovitis but more frequently associated with airsacculitis in chickens and sometimes in turkeys [2]. Both diseases are economically important, egg transmitted and hatchery disseminated diseases. They lead to tremendous economic losses in poultry production as a result of decreased hatchability and egg production, reduced quality of day-old chicks, reduced growth rate, increased costs of eradication procedures which involve site cleaning and depopulation, and increased costs of medication and vaccination [3]. The first isolation of both mycoplasmas in Sudan was reported by Khalda [4]. A recent study indicated that these organisms were prevalent, as 50.8% Mg and 57.6% Ms antibodies were recorded in chickens in the country [5]. For many years, diagnosis of avian mycoplasmosis was based on serological assays to detect antibody production and/or on isolation and identification of the organism. Serological tests include the rapid slide
%U http://www.hindawi.com/journals/jvm/2013/208026/