%0 Journal Article
%T PTPIP51: A New Interaction Partner of the Insulin Receptor and PKA in Adipose Tissue
%A M. A. Bobrich
%A S. A. Schwabe
%A A. Brobeil
%A M. Viard
%A M. Kamm
%A F. C. Mooren
%A K. Kr¨ąger
%A C. Tag
%A M. Wimmer
%J Journal of Obesity
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/476240
%X Aims. Our previous experiments revealed an association of PTPIP51 (protein tyrosine phosphatase interacting protein 51) with the insulin signalling pathway through PTP1B and 14-3-3beta. We aimed to clarify the role of PTPIP51 in adipocyte metabolism. Methods. Four groups of ten C57Bl/6 mice each were used. Two groups were fed a standard diet; two groups were fed a high-fat diet. Two groups (one high-fat diet and one standard diet) were submitted to endurance training, while the remaining two groups served as untrained control groups. After ten weeks, we measured glucose tolerance of the mice. Adipose tissue samples were analyzed by immunofluorescence and Duolink proximity ligation assay to quantify interactions of PTPIP51 with either insulin receptor (IR) or PKA. Results. PTPIP51 and the IR and PTPIP51 and PKA, respectively, were colocalized in all groups. Standard diet animals that were submitted to endurance training showed low PTPIP51-IR and PTPIP51-PKA interactions. The interaction levels of both the IR and PKA differed between the feeding and training groups. Conclusion. PTPIP51 might serve as a linking protein in adipocyte metabolism by connecting the IR-triggered lipogenesis with the PKA-dependent lipolysis. PTPIP51 interacts with both proteins, therefore being a potential gateway for the cooperation of both pathways. 1. Introduction Obesity abets several life style diseases such as diabetes type II, atherosclerosis, or impaired wound healing. These adverse effects can be avoided or, if already present, reduced by physical activity. Physical activity increases sympathetic activity and blood catecholamine levels. Adaption to periodic physical exercise is well studied. Exercise training and increased catecholamine levels affect adipocyte metabolism, on one hand by influencing insulin secretion [1] and on the other hand by additionally channelling the adipocyte metabolism towards lipolysis [2]. This effect is mediated by beta2-adrenergic receptors which activate the intracellular adenylyl cyclase. Adenylyl cyclase transforms ATP into cyclic AMP (cAMP), which in turn increases the activity level of cAMP-dependent protein kinase A (PKA) [3, 4]. Protein kinase A thereupon initiates lipolysis [4]. The most potent antagonist of catecholamines on adipose tissue is insulin. Activation of the insulin receptor results in decreased lipolysis, increased lipogenesis and uptake of fatty acids. Lipolysis is decreased by insulin-receptor-induced phosphodiesterase-3B (PDE-3B) and 14-3-3beta-dependent reduction of PKA activity [3, 5]. Furthermore, the insulin
%U http://www.hindawi.com/journals/jobe/2013/476240/