%0 Journal Article
%T Protective Effects of Resveratrol on TNF- -Induced Endothelial Cytotoxicity in Baboon Femoral Arterial Endothelial Cells
%A Juan Xiao
%A Jun Song
%A Vida Hodara
%A Allen Ford
%A Xing Li Wang
%A Qiang Shi
%A Li Chen
%A John L. VandeBerg
%J Journal of Diabetes Research
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/185172
%X Endothelial injury induced by inflammatory factors plays a critical role in the pathogenesis of cardiovascular disease. Endothelial cell (EC) apoptosis, proliferation, migration, and cellular adhesion molecule (CAM) expression contribute to the development of atherosclerosis. We investigated the effects of resveratrol (0.1¨C100£¿¦ÌM) on the proliferation, migration, and CAM expression of primary cultures of baboon arterial endothelial cells (BAECs). In addition, we tested its effects under normal conditions as well as under inflammatory conditions induced by tumour necrosis factor- (TNF- ) administered either by cotreatment, pretreatment, or posttreatment. Immunocytochemistry, MTT, wound-healing, and flow cytometry assays were performed. The resveratrol treatment significantly enhanced BAEC proliferation and attenuated TNF- -induced impairment of proliferation at the optimal doses of 1¨C50£¿¦ÌM. Resveratrol at a high dose (100£¿¦ÌM) and TNF- impaired BAEC migration, while low doses of resveratrol (1¨C50£¿¦ÌM) attenuated TNF- -induced impairment of BAEC migration. Moreover, resveratrol inhibited TNF- -induced ICAM-1 and VCAM-1 expression. Taken together, our results suggest that the resveratrol protects BAECs after inflammatory stimulation as well as ameliorates inflammatory effects at low concentrations. Consequently, resveratrol should be considered as a candidate drug for the prevention and treatment of inflammatory vascular diseases. 1. Introduction Endothelial cell (EC) cytotoxicity induced by inflammatory factors plays a key role in the pathogenesis of cardiovascular disease. Tumour necrosis factor (TNF)- , a pleiotropic proinflammatory cytokine involved in the pathogenesis of inflammatory, and vascular disease, can promote endothelial cell apoptosis and inflammation [1] by directly activating a number of cellular stress-sensitive pathways including nuclear factor-kappa B (NF- B) and mitogen-activated protein kinase (MAPK) [2]. These subsequently contribute to endothelial cell injury and cellular dysfunction [3]. Therefore, the inhibition of TNF- -induced endothelial cell cytotoxicity can be important in preventing the cardiovascular disease and inhibiting its progression. The resveratrol possesses many pharmacological properties including anticancer [4], anti-inflammation [5], and cardioprotective effects [6]. The resveratrol exerts direct cardiovascular protective effects by improving myocardial perfusion, reducing oxidant stress, and inhibiting platelet aggregation [7¨C9]. Recent studies have shown that resveratrol acts partially through the inhibition of
%U http://www.hindawi.com/journals/jdr/2013/185172/