%0 Journal Article
%T An Efficient Apparatus for Rapid Deoxygenation of Erythrocyte Concentrates for Alternative Banking Strategies
%A Lello Zolla
%A Angelo D'Alessandro
%J Journal of Blood Transfusion
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/896537
%X Erythrocyte concentrates (ECs) stored for transfusion purposes still represent a lifesaving solution in a wide series of clinically occurring circumstances, especially for traumatized and perioperative patients. However, concerns still arise and persist as to whether current criteria for collection and storage of ECs might actually represent the best case scenario or there might rather be still room for improvement. In particular, the prolonged storage of EC has been associated with the accumulation of a wide series of storage lesions, either reversible (metabolism) or irreversible (protein and morphology). Independent laboratories have contributed to propose alternative strategies, among which is the introduction of oxygen removal treatments to ECs. Convincing biochemical and preliminary clinical evidences have been produced about the benefits derived from the introduction of this practice. We, hereby, propose a rapid, efficient, and time-effective strategy for blood deoxygenation which might fit in current EC production chain. The proposed strategy resulted in the complete deoxygenation of red blood cell hemoglobin ( £¿mmHg). A preliminary small-scale study about the application of the present method resulted in reduced hemolysis, decreased vesiculation, and limited alterations to the red blood cell morphology, as gleaned from flow cytometry and scanning electron microscopic analyses. Further in-depth and larger-scale investigations are encouraged. 1. Introduction Erythrocyte concentrates (ECs) are still the most widely transfused blood-derived therapeutic worldwide, as many million units of blood are collected, and million units of RBCs are administered to millions of patients every year [1, 2]. Currently, accepted European Council guidelines indicate that ECs may be stored for up to 42 days under controlled conditions before transfusion [3]. Nevertheless, recent retrospective and controversial studies have brought about concerns on the suitability of longer stored EC units for transfusion purposes [4, 5]. It has indeed been stressed that the risk of exposure to long-stored red blood cells (RBCs) is exacerbated when dealing with certain categories of recipients, such as traumatized, postoperative, and critically ill patients [5]. However, it should be worth mentioning that early results from randomized double-blind clinical prospective trials have not hitherto indicated any statistically significant disadvantage of the administration of longer stored units in comparison to fresher blood [6, 7]. While the likelihood of untoward effects related to the
%U http://www.hindawi.com/journals/jbt/2013/896537/