%0 Journal Article
%T A Comparison Study of the Blood Component Quality of Whole Blood Held Overnight at 4¡ãC or Room Temperature
%A Shichun Wang
%A Tiantian Wang
%A Yahan Fan
%A Shan Huang
%A Zhongmei Yi
%A Ruiqing Li
%A Shuming Zhao
%J Journal of Blood Transfusion
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/523539
%X Background. The use of plasma frozen within 24£¿hrs is likely to increase. Whole blood (WB) and buffy coats (BCs) can be held for a few hrs or overnight before processing. Methods. Twenty-four bags of WB for plasma and 12 bags for platelet (PLT) concentrates were collected. The fresh frozen plasma (FFP) was prepared within 6£¿hrs. I-FP24 and II-FP24 samples were prepared either from leukodepleted WB that was held overnight or from WB that was held overnight before leukodepletion. The PLT concentrates (PCs) were prepared from BCs within 6£¿hrs (PC1) and within 18 to 24£¿hrs (PC2). The typical coagulation factors and some biochemical parameters were determined. Results. Compared to the FFP samples, the levels of FVII and FVIII in the I-FP24 and II-FP24 samples decreased significantly. The pH, Na+, LDH, and FHb levels differed significantly between II-FP24 and FFP. Compared to PC1, PC2 exhibited lower pH, pO2, and Na+ levels, a higher PLT count, and increased pCO2, K+, Lac, and CD62P expression levels. Conclusion. FP24 is best prepared from WB that was stored overnight at 4¡ãC and then leukodepleted and separated within 24£¿hrs. PCs are best produced from BCs derived from WB that was held overnight at room temperature. 1. Introduction An important step in safeguarding the quality and safety of the blood supply is recruiting volunteer donors from low-risk populations and producing qualified blood and blood components. Volunteer donor recruitment is a challenging proposition worldwide. In China, it was particularly difficult before 1998. Traditional Chinese culture believes that the loss of even a small amount of blood has a substantial detrimental health effect. Some people also believe that blood donation is a disloyal action against one¡¯s ancestors. Old cultural beliefs, combined with inadequate efforts to mobilize volunteer donors, have led to a chronic shortage of blood products in some areas of China [1]. Therefore, many blood centers have tried to prepare more blood components, such as platelet (PLT) concentrates (PCs), from whole blood (WB) to meet the need for blood products. WB units are generally held at ambient temperatures (20¨C24¡ãC) when PCs will be prepared within 6£¿hrs of the blood collection. The United States Food and Drug Administration (FDA) guidelines during the early 1980s allowed the preparation of components at 20 to 24¡ãC within 8£¿hrs of collection and a 5-day storage period for PCs. The impetus for extending the time to 8£¿hrs reflected an increased production demand for PCs and the limitations of the 6£¿hr period with respect to the
%U http://www.hindawi.com/journals/jbt/2013/523539/