%0 Journal Article
%T Effect of Addition of Melatonin on the Liquid Storage (5¡ãC) of Mithun (Bos frontalis) Semen
%A P. Perumal
%A Kezhavituo Vupru
%A K. Khate
%J International Journal of Zoology
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/642632
%X The present study was undertaken to assess the effect of melatonin (MT) on sperm motility, viability, total sperm abnormality, acrosomal and plasma membrane integrity, DNA abnormality, antioxidant profiles such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and total antioxidant capacity (TAC), enzymatic profiles such as aspartate amino transaminase (AST), alanine amino transaminase (ALT), and biochemical profiles such as malonaldehyde (MDA) production and cholesterol efflux. Total numbers of 30 ejaculates were collected twice a week from eight mithun bulls and semen was split into five equal aliquots, diluted with the TEYC extender. Group 1 has semen without additives (control) and group 2 to group 5 have semen that was diluted with 1£¿mM, 2£¿mM, 3£¿mM, and 4£¿mM of melatonin, respectively. These seminal parameters, antioxidant, enzymatic, and biochemical profiles were assessed at 5¡ãC for 0, 6, 12, 24, and 30£¿h of incubation. Inclusion of melatonin into diluent resulted in significant ( ) decrease in percentages of dead spermatozoa, abnormal spermatozoa, and acrosomal abnormalities at different hours of storage periods as compared with control group. Additionally, melatonin at 3£¿mM has significant improvement in quality of mithun semen than melatonin at 1£¿mM, 2£¿mM or 4£¿mM stored in in vitro for up to 30£¿h. It was concluded that the possible protective effects of melatonin on sperm parameters are it prevents MDA production and preserve the antioxidants and intracellular enzymes during preservation. 1. Introduction Mithun (Bos frontalis) is a semiwild free-range, rare bovine species present in the North-Eastern Hill (NEH) region of India. It is believed to have originated more than 8000 years ago from wild Indian gaur (Bos gaurus) [1]. The animal has an important place in the social, cultural, religious, and economic life of the tribal population particularly in the states of Arunachal Pradesh, Nagaland, Manipur and Mizoram. Recent statistics indicates that the mithun population is decreasing gradually due to lack of suitable breeding bulls, increase in intensive inbreeding practices, declining land area for grazing and lack of suitable breeding and feeding management in NEH region. Greater efforts are required from all quarters to preserve the mithun population to enhance the socioeconomic status of this region. Since mithuns are semiwild animal and not fully domesticated, natural breeding is practiced in this species with accompanied limitations like cost and disease transmission. Thus, use of artificial insemination for improvement of
%U http://www.hindawi.com/journals/ijz/2013/642632/