%0 Journal Article
%T Resolution of Ternary Mixture of Aspirin, Atorvastatin, and Clopidogrel by Chemometric-Assisted UV Spectroscopic and Liquid Chromatography Methods
%A Mahmoud Mohamed Issa
%A R'afat Mahmoud Nejem
%A Alaa Abu Shanab
%A Raluca-Ioana Stefan-van Staden
%J International Journal of Spectroscopy
%D 2013
%I Hindawi Publishing Corporation
%R 10.1155/2013/726820
%X Two chemometrics-assisted UV spectrophotometric methods were proposed for the resolution of ternary mixtures without any chemical pretreatment. The first method is based on modification of H-point standard addition method which permits simultaneous analysis of three species from a unique calibration set by making the simultaneous addition of the three analytes. Quotient between the spectra of aspirin, atorvastatin, and clopidogrel was obtained and the results showed that simultaneous determination of aspirin, atorvastatin, and clopidogrel can be obeyed in the linear range 2.5每20ˋ米gˋmLˋ1 of aspirin, 2.5每17.5ˋ米gˋmLˋ1 of atorvastatin, and 2.5每20ˋ米gˋmLˋ1 of clopidogrel in ternary mixture. The second method is based on the combination of the first derivative spectra and Cramer's matrix rule. In the matrix calculation, clopidogrel has zero crossing point at 316.8 and 212ˋnm, while for atorvastatin the zero crossing point at 250ˋnm where the matrix is greatly simplified and easily solved. The linear concentration ranges were 2.5每20ˋ米gˋmLˋ1 aspirin, 2.5每17.5ˋ米gˋmLˋ1 atorvastatin and 2.5每20ˋ米gˋmLˋ1 clopidogrel in ternary mixtures. The results proved that the simultaneous determination of aspirin, atorvastatin, and clopidogrel could be obeyed. Both methods were applied for capsules containing the three ingredients and results were in good concordance with alternative liquid chromatography. 1. Introduction Aspirin (ASP, acetylsalicylic acid) is used as an analgesic, antipyretic, anti-inflammatory medication and an antiplatelet agent. For clopidogrel bisulphate (CLOP), chemically it is methyl (+)-(S)-汐-(2-chlorophenyl)-6,7-dihydrothieno[3, 2-c]pyridine-5(4H)acetate sulfate (1ˋ:ˋ1) and is an oral, thienopyridine class antiplatelet agent used to inhibit blood clots in coronary artery disease, peripheral vascular disease, and cerebrovascular disease. Atorvastatin calcium (ATOR), [R- ]-2-(4-fluorophenyl)-汕,汛,-dihydroxy-5-(1-methylethyl)-3-phenyl-4 [(phenylamino)carbonyl]-lH-pyrrole-1-heptanoic acid, calcium salt (2ˋ:ˋ1) trihydrate is an inhibitor of 3 hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase. Chemical structures of ASP, CLOP, and ATOR are shown in Scheme 1. Scheme 1: Aspirin (a), clopidogrel (b), and atorvastatin (c). A combination of ASP, CLOP, and ATOR drugs is available in the market as capsules. The ternary combination is used for atherosclerotic patients suffering from various heart diseases. Clinical trials showed that combination therapy when used in dyslipidaemic patient with coronary heart disease reduced cardiovascular events. The official
%U http://www.hindawi.com/journals/ijs/2013/726820/