%0 Journal Article
%T Clinical Feature of Intrahepatic B-Lymphocytes in Chronic Hepatitis B
%A Ashraf Mohamadkhani
%A Elnaz Naderi
%A Masoud Sotoudeh
%A Aezam Katoonizadeh
%A Ghodratollah Montazeri
%A Hossein Poustchi
%J International Journal of Inflammation
%D 2014
%I Hindawi Publishing Corporation
%R 10.1155/2014/896864
%X Humoral immunity constitutes major defense mechanism against viral infections. However, the association of hepatic injury and B-cells population in chronic hepatitis B virus (HBV) carriers has not been studied well. In this study, fifty seven hepatitis B surface antigen (HBsAg) positive and HBeAg negative patients were studied to determine the expression of CD20, a cell surface marker expressed on B-cells, in liver biopsy sections using immunohistochemistry. The patients¡¯ clinical data at the time of liver biopsy were acquired from their medical records. There was a significant association between log HBV DNA and both ALT ( , ) and histologic activity index (HAI) total score ( , ), respectively. The CD20 was expressed in all 57 liver biopsy samples with a submembranous and membranous staining pattern and its expression was significantly associated with HAI total score ( , ) and stage of fibrosis ( , ). The susceptible B lymphocytes to hepatitis B virus might be implicated in the development of immune mediated inflammation of HBV-induced hepatic injury. The present data also support that the liver is potentially one of the secondary lymphoid organs. 1. Introduction Chronic hepatitis B (CHB) virus (HBV) infection is the principal cause of cirrhosis and hepatocellular carcinoma (HCC) [1]. The pathogenesis of HBV-related chronic liver disease is not well understood. However, it is clear that the immune mechanisms associated with the antiviral response are responsible for CHB outcome [2¨C4]. The existence of lymphocytes in the human liver is representing a pathological situation [5]. This concept stems from the observation that, in chronic hepatitis B, T-cells can potentially participate both in the immune clearance of HBV-infected cells and in the pathogenesis of hepatocellular injury [6]. Furthermore the numbers of B lymphocytes and plasma cells are significantly higher in patients with liver cirrhosis than of those with inactive chronic hepatitis [7, 8]. Enormous intrahepatic B-cells with massive production of IgM and IgG and infiltrating plasma cells into the hepatic lobules have also been shown in HBV-associated chronic active hepatitis [9]. B-cells contribute to immune responses through the secretion of effector cytokines and it has been suggested that naive and memory B-cell subsets preferentially produce different effector cytokines [10, 11]. Na£¿ve B-cells undergo maturation by somatic hypermutation in immunoglobulin variable region of the B-cell receptor (BCR) genes following contact with a specific protein accessible on dendritic cells. Then the
%U http://www.hindawi.com/journals/iji/2014/896864/