%0 Journal Article
%T Cytoplasmic Ribonucleoprotein Foci in Eukaryotes: Hotspots of Bio(chemical)Diversity
%A Carla Layana
%A Paola Ferrero
%A Rolando Rivera-Pomar
%J International Journal of Genomics
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/504292
%X The life of an mRNA from transcription to degradation offers multiple control check points that regulate gene expression. Transcription, splicing, and translation have been widely studied for many years; however, in recent years, new layers of posttranscriptional and posttranslational control have been uncovered. They involve the regulation of the metabolism of mRNA in cytoplasmic foci. They are collections of ribonucleoprotein complexes that, in most cases, remain still uncharacterized, except the processing bodies (PBs) and stress granules (SGs), which have been studied (and reviewed) in detail. A challenging prospective is to know how many different classes of foci exist, which functions they support, how are they formed, and how do they relate one to each other. Here, we present an update of the component of the different granules, a possible function, and hypothesis on their in vivo dynamics related to translational control. 1. Introduction In recent years, several cytoplasmic foci/granules that contain proteins and RNA have been described. Two of them have been studied in more detail as they are related to mRNA silencing: stress granules (SG) and processing bodies (PB). SG are repressed mRNPs transiently induced in response to cellular stress. They range from 0,5 to 5£¿¦Ìm [1]. PB are discrete RNP cytoplasmic foci of 0,1-2£¿¦Ìm where the machinery of RNA interference, degradation and storage locates. In PB the mRNAs are forming mRNP complexes either repressing translation, in degradation complexes or stored for further use [2, 3]. SG and PB have been shown to share a growing number of proteins that are added in a day-to-day basis to the list of their components. SG, PB and other cytoplasmic foci are highly dynamic structures, although PB are quite stable over the time [4]; see also Supplementary Movie 1 available online at http://dx.doi.org/10.1155/2012/504292. They are in a dynamic steady state with other mRNPs, such as polysomes in response to the translational state of the cell [5]. Although we do not intend to extensively review SG and PB, which have been matter of fine reviews in the last years [6¨C10], we will overview their functions before we address neglected issues and hypothesis. 2. Stress Granules Translation initiation is the key regulatory step of translational control. Therefore, it is the most sensitive step to changes in the cellular environment, including stress. A key step in translation initiation inhibition is the phosphorylation of eIF2¦Á, which results in an increase on the affinity of eIF2-GDP for eIF2B, sequestering this factor
%U http://www.hindawi.com/journals/ijg/2012/504292/