%0 Journal Article
%T Novel Evolutionary Models and Periodic Charts in p- and q-Individual Chromosomes of Auxiliary Lymph Node and Buccal Cells
%A P. Mehdipour
%A F. Javan
%A M. Atri
%J Disease Markers
%D 2013
%R 10.1155/2013/570946
%X Signal copy number (SCN) and signal intensity (SI) of subtelomeres (ST) are investigated in auxiliary lymph node (ALN) and buccal (BUC) cells by fluorescence in situ hybridization. The extracted total cell of 38256 and 2309 was, respectively, analyzed from the benign ALN- and BUC-cells of an affected breast cancer patient. The Periodic model was based on ST behavior including normal-, down-, and upregulated clones with diverse SCN. The arm-p/q ratio based signature, as a subtelomeric array, reflects discordance and concordance of ST-behavior within individual chromosomes as a concept of ¡°Individualization of Cells¡± rather than ¡°Global Insight of Cells¡±. The Periodic charts could be considered as a reliable and refreshable platform through which the cellular evolution could be patterned and characterized. Signature of ST-profile in the BUC and ALN cells and the nature of diverse SCN and SI as quantitative and qualitative value led to modeling the real personalized perspective of cellular evolution. Protein expression of Ki67, Cyclin D1, and Cyclin E was assayed, as a complementary panel. These targets could be applied as the predictive and preventive markers for an early detection at BUC and ALN levels to plan the required managements in the breast cancer patients. Modeling such signature and mining the subtelomeric signal profiling of individual chromosomes' arms could be translated to a personalized developmental insight in buccal-cells and auxiliary lymph node of breast neoplasm¡¯s cells by Novel Periodic Charts. 1. Introduction The reliability of buccal (BUC) cells is due to its sensitivity to different substances used by people who may be affected with cancer or other diseases. In such process, the materials in the BUC are combined with dietary factors, and/or any environmental hazard elements (http://aghealth.nih.gov/ 2002). The mucous-secreting epithelial, known as mucosa, is classified into two types: (1) masticatory (keratinized) and (2) inside layer (nonkeratinized). The masticatory mucosa appears to be thick, with a denser and less vascular connective tissue component. Keratin acts as a shielding domain against different types of stimuli [1]. BUC cells are, morphologically, characterized with their relatively large size, flat shape, nongranular cytoplasm, centerline located nucleus, and large ratio of cytoplasm to nucleus. Such characteristics make BUC a reliable tissue for investigations in cell biology and genetics. Unmasking the nature of diverse copy number as quantitative value and mode of intensity as qualitative insight could help to
%U http://www.hindawi.com/journals/dm/2013/570946/