%0 Journal Article
%T In Vivo Antioxidant Activity of Topical Cream of Cassia tora L. Leaves Extract
%A V. Gupta
%A D. S. Rathore
%A Niraj P. Kansara
%A A. M. Badiger
%J Dataset Papers in Science
%D 2013
%R 10.7167/2013/243949
%X Objective. The aim of study was to determine the in vivo antioxidant activity of newly formulated O/W cream of methanolic extract of Cassia tora L. leaves. Methods. Oil in water (O/W) creams (0.05%, 0.1%, and 0.2%), cream base, methanolic extract of C. tora L. leaves (CTM), and standard (0.05% tretinoin cream) were screened for in vivo antioxidant activity. The ultraviolet- (UV-) B-induced rats were treated with different standard, O/W creams, cream base, and methanolic extract of Cassia tora L. leaves (CTM). The parameters like lipid peroxidation (LPO), reduced glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) levels were assessed. Results. The result of acute dermal toxicity shows that the creams were safe up to 2000£¿mg/kg (topically). Exposure of ultraviolet light of medium wave (UV-B light) decreased the level of GSH, CAT, and SOD and increased the LPO level. Concluding Remarks. We conclude that topical O/W creams of C. tora L. prevent the oxidative stress induced in rats by exposure to UV-B light by virtue of its in vivo antioxidant property, and these findings help to understand the mechanism of the antipsoriatic activity of O/W creams of C. tora L. in UV-B-induced psoriasis in the rat. 1. Introduction N. P. Kansara found a potent antipsoriatic activity of O/W creams of Cassia tora L. in ultraviolet- (UV) -B-induced psoriasis in the rat. Aerobic organisms produce reactive oxygen species during normal respiration and inflammatory conditions. Exercise can create an imbalance between oxidant and antioxidant levels, a situation known as oxidative stress. Indeed, oxidative stress resulting from acute exercise in unadapted and adapted subjects has been proposed to damage enzymes, protein receptors, lipid membranes, and DNA [1].The plant C. tora L. (Caesalpiniaceae) is traditionally claimed to be useful in the treatment of psoriasis and other skin diseases [2, 3]. C. tora L. leaves enriched in glycosides and also containing aloe-emodin may be beneficial for the skin diseases [4]. C. tora L. has been reported to possess a significant antioxidant activity in in vitro activity by using DPPH (2,2¡ä-diphenyl-1-picryhydrazyl) method [5]. In these antioxidant assays, the volatile oil from C. tora L. demonstrated 2,2¡ä-diphenyl-1-picryhydrazyl radical-scavenging activity in the concentration range from 20 to 500£¿¦Ìg/mL, with the 50% inhibitory concentration (IC50) value at 137£¿¦Ìg/mL; it also showed a significant inhibitory effect against hydroxyl radicals with an IC50 value of 67£¿¦Ìg/mL, lower than that of quercetin (IC50 = 8.15£¿¦Ìg/mL), but superior
%U http://www.hindawi.com/journals/dpis/2013/243949/