%0 Journal Article
%T Elevated MiR-222-3p Promotes Proliferation and Invasion of Endometrial Carcinoma via Targeting ER¦Á
%A Binya Liu
%A Qi Che
%A Haifeng Qiu
%A Wei Bao
%A Xiaoyue Chen
%A Wen Lu
%A Bilan Li
%A Xiaoping Wan
%J PLOS ONE
%D 2014
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0087563
%X MicroRNAs play key roles in tumor proliferation and invasion. Here we show distinct expression of miR-222-3p between ER¦Á-positive and ER¦Á-negative endometrial carcinoma (EC) cell lines and primary tumors, and investigation of its relationship with ER¦Á and other clinical parameters. In vitro, the function of miR-222-3p was examined in RL95-2 and AN3CA cell lines. MiR-222-3p expression was negatively correlated with ER¦Á. Over-expressed miR-222-3p in RL95-2 cells promoted cell proliferation, enhanced invasiveness and induced a G1 to S phase shift in cell cycle. Furthermore, the miR-222-3p inhibitor decreased the activity of AN3CA cells to proliferate and invade. In vivo, down-regulated miR-222-3p of AN3CA cells inhibited EC tumor growth in a mouse xenograft model. Additionally, miR-222-3p increased raloxifene resistance through suppressing ER¦Á expression in EC cells. In conclusion, miR-222-3p plays a significant role in the regulation of ER¦Á expression and could be potential targets for restoring ER¦Á expression and responding to antiestrogen therapy in a subset of ECs.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0087563