%0 Journal Article
%T Microrna 130b Suppresses Migration and Invasion of Colorectal Cancer Cells through Downregulation of Integrin ¦Â1
%A Yanyang Zhao
%A Gang Miao
%A Yao Li
%A Tomoya Isaji
%A Jianguo Gu
%A Jian Li
%A Ruomei Qi
%J PLOS ONE
%D 2014
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0087938
%X MicroRNA 130b (miR-130b) is significantly dysregulated in various human tumor types. In this study, using a microarray assay, we characterized the upregulation of miR-130b expression in colorectal cancer (CRC) specimens. However, there is limited knowledge about the roles of aberrant miR-130b expression in CRC. Our studies in CRC cells demonstrated that miR-130b significantly decreases cell migration and invasion, but it has no evidently effects on cell proliferation and apoptosis. In the overexpression miR-130b CRC cells and the CRC specimens, we observed a decreased level of integrin ¦Â1 protein, which is considered as a key molecule involved in cell motility. The targeting of the 3¡ä-UTR region of integrin ¦Â1 gene by miR-130b was revealed using a luciferase reporter assay. The regulation of integrin ¦Â1 by miR-130b was further shown using the miR-130b mimics and the inhibitor of miR-130b. The impaired motility of the miR-130b overexpression cells is recovered partly by the expression of integrin ¦Â1 lacking the 3¡ä-UTR. Additionally, the knockdown of integrin ¦Â1 also gives rise to a decrease in cell migration and invasion, which is similar to the impeded motility due to overexpression of miR-130b in CRC cells. Furthermore, the inverse expressions of miR-130b and integrin ¦Â1 were observed in CRC specimens. In summary, these data demonstrate that miR-130b downregulates its target-integrin ¦Â1, leading to the impaired migration and invasion of CRC cells.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0087938