%0 Journal Article
%T Growth and Nitrogen Uptake Kinetics in Cultured Prorocentrum donghaiense
%A Zhangxi Hu
%A Shunshan Duan
%A Ning Xu
%A Margaret R. Mulholland
%J PLOS ONE
%D 2014
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0094030
%X We compared growth kinetics of Prorocentrum donghaiense cultures on different nitrogen (N) compounds including nitrate (NO3ˋ), ammonium (NH4+), urea, glutamic acid (glu), dialanine (diala) and cyanate. P. donghaiense exhibited standard Monod-type growth kinetics over a range of N concentraions (0.5每500 米mol N Lˋ1 for NO3ˋ and NH4+, 0.5每50 米mol N Lˋ1 for urea, 0.5每100 米mol N Lˋ1 for glu and cyanate, and 0.5每200 米mol N Lˋ1 for diala) for all of the N compounds tested. Cultures grown on glu and urea had the highest maximum growth rates (米m, 1.51㊣0.06 dˋ1 and 1.50㊣0.05 dˋ1, respectively). However, cultures grown on cyanate, NO3ˋ, and NH4+ had lower half saturation constants (K米, 0.28每0.51 米mol N Lˋ1). N uptake kinetics were measured in NO3ˋ-deplete and -replete batch cultures of P. donghaiense. In NO3ˋ-deplete batch cultures, P. donghaiense exhibited Michaelis-Menten type uptake kinetics for NO3ˋ, NH4+, urea and algal amino acids; uptake was saturated at or below 50 米mol N Lˋ1. In NO3ˋ-replete batch cultures, NH4+, urea, and algal amino acid uptake kinetics were similar to those measured in NO3ˋ-deplete batch cultures. Together, our results demonstrate that P. donghaiense can grow well on a variety of N sources, and exhibits similar uptake kinetics under both nutrient replete and deplete conditions. This may be an important factor facilitating their growth during bloom initiation and development in N-enriched estuaries where many algae compete for bioavailable N and the nutrient environment changes as a result of algal growth.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0094030