%0 Journal Article
%T Study on the Interaction of ¦Â-Cyclodextrin and Berberine Hydrochloride and Its Analytical Application
%A Baoxiu Jia
%A Yuqin Li
%A Decai Wang
%A Rui Duan
%J PLOS ONE
%D 2014
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0095498
%X The fluorescence enhancement of berberine hydrochloride (BBH) as a result of complex with ¦Â-cyclodextrin (¦Â-CD) is investigated. The mechanism of the inclusion was studied and discussed by spectrofluoremetry and infrared spectrograms. The results showed that a 1:1 (¦Â-CD: BBH) complex was formed with an apparent association constant of 4.23¡Á102 L/mol. Based on the enhancement of the fluorescent intensity of berberine hydrochloride, a new spectrofluorimetric method for the determination of BBH in the presence of ¦Â-CD was developed. The linear range was 1.00~4.00 ¦Ìg/mL with the detection limit of 5.54 ng/mL. The proposed method was successfully applied to the determination of BBH in tablets.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0095498