%0 Journal Article
%T Description of the L76V Resistance Protease Mutation in HIV-1 B and ¡°Non-B¡± Subtypes
%A Charlotte Charpentier
%A Sidonie Lambert-Niclot
%A Claudia Alteri
%A Alexandre Storto
%A Philippe Flandre
%A Valentina Svicher
%A Carlo-Federico Perno
%A Fran£¿oise Brun-V¨¦zinet
%A Vincent Calvez
%A Anne-Genevi¨¨ve Marcelin
%A Francesca Ceccherini-Silberstein
%A Diane Descamps
%J PLOS ONE
%D 2013
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0054381
%X Objective To describe the prevalence of the L76V protease inhibitors resistance-associated mutation (PI-RAM) in relation with patients¡¯ characteristics and protease genotypic background in HIV-1 B- and ¡°non-B¡±-infected patients. Methods Frequency of the L76V mutation between 1998 and 2010 was surveyed in the laboratory database of 3 clinical centers. Major PI-RAMs were identified according to the IAS-USA list. Fisher¡¯s and Wilcoxon tests were used to compare variables. Results Among the overall 29,643 sequences analyzed, the prevalence of L76V was 1.50%, while was 5.42% in PI-resistant viruses. Since 2008 the prevalence of L76V was higher in ¡°non-B¡±-infected than in B-infected patients each year. Median time since diagnosis of HIV-1 infection and median time under antiretroviral-based regimen were both shorter in ¡°non-B¡±- than in B-infected patients (8 vs 11 years, P<0.0001; and 7 vs 8 years, P = 0.004). In addition, ¡°non-B¡±-infected patients had been pre-exposed to a lower number of PI (2 vs 3, P = 0.016). The L76V was also associated with a lower number of major PI-RAMs in ¡°non-B¡± vs B samples (3 vs 4, P = 0.0001), and thus it was more frequent found as single major PI-RAM in ¡°non-B¡± vs B subtype (10% vs 2%, P = 0.014). Conclusions We showed an impact of viral subtype on the selection of the L76V major PI-RAM with a higher prevalence in ¡°non-B¡± subtypes observed since 2008. In addition, in ¡°non-B¡±-infected patients this mutation appeared more rapidly and was associated with less PI-RAM.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0054381