%0 Journal Article
%T The Oncogenic Role of microRNA-130a/301a/454 in Human Colorectal Cancer via Targeting Smad4 Expression
%A Lin Liu
%A Jing Nie
%A Lin Chen
%A Guanglong Dong
%A Xiaohui Du
%A Xin Wu
%A Yun Tang
%A Weidong Han
%J PLOS ONE
%D 2013
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0055532
%X Transforming growth factor (TGF)-¦Â/Smad signaling plays an important role in colon cancer development, progression and metastasis. In this study we demonstrated that the microRNA-130a/301a/454 family is up-regulated in colon cancer tissues compared to paired adjacent normal mucosa, which share the same 3¡ä-untranslational region (3¡ä-UTR) binding seed sequence and are predicated to target Smad4. In colorectal cancer HCT116 and SW480 cells, overexpression of miRNA-130a/301a/454 mimics enhances cell proliferation and migration, while inhibitors of these miRNAs affect cell survival. The biological function of miRNA-130a/301a/454 on colon cancer cells is likely mediated by suppression of Smad4, and the up-regulation of the miRNAs is correlated with Smad4 down-regulation in human colon cancers. Collectively, these results suggest that miRNA-130a/301a/454 are novel oncogenic miRNAs contributing to colon tumorigenesis by regulating TGF-¦Â/Smad signaling, which may have potential application in cancer therapy.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0055532