%0 Journal Article %T The Oncogenic Role of microRNA-130a/301a/454 in Human Colorectal Cancer via Targeting Smad4 Expression %A Lin Liu %A Jing Nie %A Lin Chen %A Guanglong Dong %A Xiaohui Du %A Xin Wu %A Yun Tang %A Weidong Han %J PLOS ONE %D 2013 %I Public Library of Science (PLoS) %R 10.1371/journal.pone.0055532 %X Transforming growth factor (TGF)-¦Â/Smad signaling plays an important role in colon cancer development, progression and metastasis. In this study we demonstrated that the microRNA-130a/301a/454 family is up-regulated in colon cancer tissues compared to paired adjacent normal mucosa, which share the same 3¡ä-untranslational region (3¡ä-UTR) binding seed sequence and are predicated to target Smad4. In colorectal cancer HCT116 and SW480 cells, overexpression of miRNA-130a/301a/454 mimics enhances cell proliferation and migration, while inhibitors of these miRNAs affect cell survival. The biological function of miRNA-130a/301a/454 on colon cancer cells is likely mediated by suppression of Smad4, and the up-regulation of the miRNAs is correlated with Smad4 down-regulation in human colon cancers. Collectively, these results suggest that miRNA-130a/301a/454 are novel oncogenic miRNAs contributing to colon tumorigenesis by regulating TGF-¦Â/Smad signaling, which may have potential application in cancer therapy. %U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0055532