%0 Journal Article
%T Potential Roles of CCR5+ CCR6+ Dendritic Cells Induced by Nasal Ovalbumin plus Flt3 Ligand Expressing Adenovirus for Mucosal IgA Responses
%A Yoshiko Fukuyama
%A Daisuke Tokuhara
%A Shinichi Sekine
%A Kazuyoshi Aso
%A Kosuke Kataoka
%A Julia Davydova
%A Masato Yamamoto
%A Rebekah S. Gilbert
%A Yuka Tokuhara
%A Keiko Fujihashi
%A Jun Kunisawa
%A Yoshikazu Yuki
%A Hiroshi Kiyono
%A Jerry R. McGhee
%A Kohtaro Fujihashi
%J PLOS ONE
%D 2013
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0060453
%X We assessed the role of CCR5+/CCR6+/CD11b+/CD11c+ dendritic cells (DCs) for induction of ovalbumin (OVA)-specific antibody (Ab) responses following mucosal immunization. Mice given nasal OVA plus an adenovirus expressing Flt3 ligand (Ad-FL) showed early expansion of CCR5+/CCR6+/CD11b+/CD11c+ DCs in nasopharyngeal-associated lymphoid tissue (NALT) and cervical lymph nodes (CLNs). Subsequently, this DC subset became resident in submandibular glands (SMGs) and nasal passages (NPs) in response to high levels of CCR-ligands produced in these tissues. CD11b+/CD11c+ DCs were markedly decreased in both CCR5£¿/£¿ and CCR6£¿/£¿ mice. Chimera mice reconstituted with bone marrow cells from CD11c-diphtheria toxin receptor (CD11c-DTR) and CCR5£¿/£¿ or CD11c-DTR and CCR6£¿/£¿ mice given nasal OVA plus Ad-FL had elevated plasma IgG, but reduced IgA as well as low anti-OVA secretory IgA (SIgA )Ab responses in saliva and nasal washes. These results suggest that CCR5+CCR6+ DCs play an important role in the induction of Ag-specific SIgA Ab responses.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0060453