%0 Journal Article
%T A Tet-On System for DRD1-Expressing Cells
%A Fayi Yao
%A Paul D. Walker
%A Robert G. MacKenzie
%J PLOS ONE
%D 2013
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0072681
%X Cells expressing the dopamine D1 receptor (DRD1) have significant functional roles in diverse physiological processes including locomotion and drug addiction. The present work presents a novel in vivo DRD1-Bacterial Artificial Chromosome (BAC) Tet-on system allowing for the inducible activation of tet-operated transgenes specifically within DRD1-expressing cells of transgenic mice. It is shown that the DRD1-rtTA BAC-driven expression of a tet-operated reporter is under tight regulation by doxycycline and is restricted to DRD1-expressing brain regions. The model will be a useful research tool in studies of movement and reward and associated pathologies such as Parkinson¡¯s disease and addiction.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0072681