%0 Journal Article
%T Optogenetic Control of Transcription in Zebrafish
%A Hongtao Liu
%A Gustavo Gomez
%A Sophia Lin
%A Shuo Lin
%A Chentao Lin
%J PLOS ONE
%D 2012
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0050738
%X Light inducible protein-protein interactions are powerful tools to manipulate biological processes. Genetically encoded light-gated proteins for controlling precise cellular behavior are a new and promising technology, called optogenetics. Here we exploited the blue light-induced transcription system in yeast and zebrafish, based on the blue light dependent interaction between two plant proteins, blue light photoreceptor Cryptochrome 2 (CRY2) and the bHLH transcription factor CIB1 (CRY-interacting bHLH 1). We demonstrate the utility of this system by inducing rapid transcription suppression and activation in zebrafish.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0050738