%0 Journal Article
%T Effect of Maltose Concentration on Plant Regeneration of Anther Culture with Different Genotypes in Rice (<i>Oryza sativa </i>L.)
%A Seul Gi Park
%A Mohammad Ubaidillah
%A Kyung-Min Kim
%J American Journal of Plant Sciences
%P 2265-2270
%@ 2158-2750
%D 2013
%I Scientific Research Publishing
%R 10.4236/ajps.2013.411279
%X <p class=\"MsoNormal\">
	<span lang=\"EN-US\" style=\"font-family:Verdana;\">This study
describes the impact of different concentrations of</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\"><span style=\"font-family:Verdana;\">maltose on plant
regeneration of anther culture for five genotypes of rice (</span><i><span style=\"font-family:Verdana;\">Oryza</span></i></span><i><span lang=\"EN-US\"> </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">sativa</span></i><span lang=\"EN-US\"><span style=\"font-family:Verdana;\">). N</span><sub><span style=\"font-family:Verdana;\">6</span></sub><span style=\"font-family:Verdana;\"> medium
was used for calli induction, while N</span><sub><span style=\"font-family:Verdana;\">6</span></sub><span style=\"font-family:Verdana;\"> medium supplemented with
different concentrations of maltose, 2.0 mg/L NAA and 0.5 mg/L kinase was used
for plant regeneration.</span></span><span lang=\"EN-US\"> </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">The result showed that during the</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">initial
stages of calli induction the anther cultures had varying rates of calli
formation among genotypes, with the best frequency being observed for Dreami2/CaMsrB2-8-DH-1
with a calli frequency of 27.8%.</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">Different genotypes of rice cultured
in regeneration media showed</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">varying plantlet regeneration on
media supplemented with different concentrations of</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">maltose, with low
concentrations (0.04</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">g/L) leading to</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">low frequency regeneration</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">plantlet but
high green plant production. Indeed, when Dreami2/CaMsrB2-8-DH-2 and
Dreami2/CaMsrB2-8-DH-5 were cultivated under these conditions, 100% green
plants were observed. Another</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\" style=\"font-family:Verdana;\">genotype also showed a small rate of
albino frequency in response to the lowest concentration of maltose, while
increased maltose concentrations</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\"
%K Anther Culture
%K Maltose
%K &lt
%K i&gt
%K Oryza sativa&lt
%K /i&gt
%K Plant Regeneration
%K Rice
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=39908