%0 Journal Article
%T Impact of <i>Celosia cristata</i> Extract on Adipogenesis of Native Human CD34<sup>+</sup>/CD31<sup>-</sup> Cells
%A Richard Fitoussi
%A David Est¨¨ve
%A Anne-Sophie Delassus
%A Katell Vi¨¦
%J Journal of Cosmetics, Dermatological Sciences and Applications
%P 55-63
%@ 2161-4512
%D 2013
%I Scientific Research Publishing
%R 10.4236/jcdsa.2013.33A2013
%X <p>
	<b><span style=\"font-family:Verdana;\">Background</span></b><b><span style=\"font-family:Verdana;\">: </span></b><span><span style=\"font-family:Verdana;\">The adipose tissue mainly consists of adipocytes but also contains non-adipose cells. Among them, progenitor cells represent a local pool of immature cells that,</span><i><span style=\"font-family:Verdana;\"> in vitro</span></i><span style=\"font-family:Verdana;\">, can undergo various lineage differentiation processes. These cells are thought to contribute to normal homeostasis of the adipose tissue through adipogenesis but also to the growth of the adipose tissue under chronic energy overload.</span></span><span> </span><span><span style=\"font-family:Verdana;\">The aim of the present study is to evaluate </span><i><span style=\"font-family:Verdana;\">in vitro</span></i><span style=\"font-family:Verdana;\"> the capacity of a </span><i><span style=\"font-family:Verdana;\">Celosia cristata</span></i><span style=\"font-family:Verdana;\"> extract to impact the adipogenic potential of native human adipose tissue progenitor cells, </span><i><span style=\"font-family:Verdana;\">i.e.</span></i><span style=\"font-family:Verdana;\"> commitment and differentiation towards adipogenic lineage.</span></span><span> </span><b><span style=\"font-family:Verdana;\">Methods</span></b><b><span style=\"font-family:Verdana;\">: </span></b><span><span style=\"font-family:Verdana;\">Native adipose tissue progenitor cells were isolated by immunoselection/depletion approaches from human subcutaneous adipose tissues. Two distinct cell culture conditions were used to assess the effect of </span><i><span style=\"font-family:Verdana;\">Celosia cristata</span></i><span style=\"font-family:Verdana;\"> extract on commitment and differenciation of progenitor cells. Cells were cultured either in differentiation medium for 10</span></span><span> </span><span><span style=\"font-family:Verdana;\">days in the presence/absence of </span><i><span style=\"font-family:Verdana;\">Celosia cristata</span></i><span style=\"font-family:Verdana;\"> extracts to study the impact on differentiation or first cultured in a commitment-inducing medium, with or without </span><i><span style=\"font-family:Verdana;\">Celosia cristata</span></i><span style=\"font-family:Verdana;\"> extract, for 48</span></span><span> </span><span style=\"font-family:Verdana;\">h and then cultured 10 days in differentiation medium to assess the impact on commitment. In both experimental series, the fate of progenitor cells was studied by quantification of lipids and by
%K Adipose Tissue
%K Stem/Progenitor Cells
%K Adipogenic Commitment
%K Adipogenic Differentiation
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=40088