%0 Journal Article
%T Effect of Cold-Mediated Pretreatment on Microspore Culture in Winter and Spring Wheat
%A Rituraj Khound
%A Meenakshi Santra
%A P. Stephen Baenziger
%A Dipak K. Santra
%J American Journal of Plant Sciences
%P 2259-2264
%@ 2158-2750
%D 2013
%I Scientific Research Publishing
%R 10.4236/ajps.2013.411278
%X <p>
	<span style=\"font-family:Verdana;\">Microspore culture of wheat generates completely
homozygous (doubled haploid) plants in a single generation thereby reducing the
time required for wheat variety development. Success of microspore culture in
spring wheat is relatively higher than</span><span style=\"font-family:Verdana;\"> that in</span><span style=\"font-family:Verdana;\"> winter wheat. Cold
mediated pretreatment was reported to improve response of microspore culture in
wheat. The objective of the study was to determine and compare the influence of
cold pretreatment on microspore cul</span><span style=\"font-family:Verdana;\">ture in spring and winter wheat.
Three spring (</span><span style=\"font-family:Verdana;\">¡°</span><span style=\"font-family:Verdana;\">Chris</span><span style=\"font-family:Verdana;\">¡±</span><span style=\"font-family:Verdana;\">, </span><span style=\"font-family:Verdana;\">¡°</span><span style=\"font-family:Verdana;\">Express</span><span style=\"font-family:Verdana;\">¡±</span><span style=\"font-family:Verdana;\">, and </span><span style=\"font-family:Verdana;\">¡°</span><span style=\"font-family:Verdana;\">Macon</span><span style=\"font-family:Verdana;\">¡±</span><span style=\"font-family:Verdana;\">) and three winter (</span><span style=\"font-family:Verdana;\">¡°</span><span style=\"font-family:Verdana;\">Anton</span><span style=\"font-family:Verdana;\">¡±</span><span style=\"font-family:Verdana;\">, </span><span style=\"font-family:Verdana;\">¡°</span><span style=\"font-family:Verdana;\">Ante</span><span style=\"font-family:Verdana;\">lope</span><span style=\"font-family:Verdana;\">¡±</span><span style=\"font-family:Verdana;\">, and </span><span style=\"font-family:Verdana;\">¡°</span><span style=\"font-family:Verdana;\">Camelot</span><span style=\"font-family:Verdana;\">¡±</span><span style=\"font-family:Verdana;\">) wheat cultivars were
used. In cold pretreatment, excised anthers were incubated in solution B at 25<span style=\"font-family:Verdana, Helvetica, Arial;white-space:normal;background-color:#FFFFFF;\">¡ã</span>C-</span><span style=\"font-family:Verdana;\">28<span style=\"font-family:Verdana, Helvetica, Arial;white-space:normal;background-color:#FFFFFF;\">¡ã</span>C for 4</span><span style=\"font-family:Verdana;\">-</span><span style=\"font-family:Verdana;\">5 days followed by cold treatment at 4<span style=\"font-family:Verdana, Helvetica, Arial;white-space:normal;background-color:#FFFFFF;\">¡ã</span>C for 5 days and </span><span style=\"font-family:Verdana;\">were</span><span style=\"font-family:Verdana;\"> compared
%K Androgenesis
%K Doubled Haploid
%K Homozygosity
%K Biotechnology
%K Breeding
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=39655