%0 Journal Article
%T MG289 in <i>Mycoplasma genitalium</i> Enhances Microbial  Invasion and Bacterial Persistence  in Benign Human Prostate Cells
%A Wasia Rizwani
%A Leticia Reyes
%A Jeongsoon Kim
%A Steve Goodison
%A Charles J. Rosser
%J Open Journal of Urology
%P 232-245
%@ 2160-5629
%D 2013
%I Scientific Research Publishing
%R 10.4236/oju.2013.36044
%X <p class=\"MsoNormal\">
	<strong><span style=\"font-family:Verdana;\">Introduction:</span></strong><span style=\"font-family:Verdana;\"> Recent studies suggest that infectious organisms may facilitate
initiation and metastasis of many human cancers. One infectious organism of
interest is <span style=\"white-space:nowrap;\"><i>Mycoplasma genitalium</i></span>(Mg),
a prevalent organism in humans known to cause sexually transmitted infection,
as well as urethritis and prostatitis. Previous studies have demonstrated that
benign, non-tumorigenic human prostate cells (BPH-1) chronically exposed to M. genitalium led to the malignant
transformation of these cells as demonstrated in in vitro and in vivo models. Based on work from our laboratory, we felt this malignant
transformation revolved around a specific M.
genitalium¡¯s ABC transporter (MG289) with homology to M. hyorhinis¡¯ ABC
transporter, p37. In this study, differences in M. genitalium¡¯s ability to infect and induce a unique proteome
conducive to tumoral growth were studied with engineered M. genitalium in which the p37 protein was silent. </span><strong><span style=\"font-family:Verdana;\">Materials and Methods:</span></strong><span style=\"font-family:Verdana;\"> Wild-type M. genitalium (strain </span><st1:chmetcnv tcsc=\"0\" numbertype=\"1\" negative=\"False\" hasspace=\"False\" sourcevalue=\"431\" unitname=\"C\" w:st=\"on\"><span style=\"font-family:Verdana;\">431c</span></st1:chmetcnv><span style=\"font-family:Verdana;\">, designated as M. genitalium WT) and MG289 deficient M. genitalium mutant (strain 260_3, designated as Mg260_3) were
used for this study. We studied the infectivity potential between M. genitalium WT and Mg260_3 upon
exposure to BPH-1 cells. Furthermore, we set out to identify a unique proteome
in BPH-1 cells exposed to M. genitalium WT that could explain its ability to induce malignant transformation of benign
cells. Validation of selected proteomic targets was carried out by Western
blot analysis. </span><strong><span style=\"font-family:Verdana;\">Results:</span></strong><span style=\"font-family:Verdana;\"> Both M. genitalium WT and
Mg260_3 strains showed somewhat similar growth curve when absorbance at 450nm
was matched at day 0. Colony forming units (CFUs) were similar for both strains
at the same absorbance. However, the ability to infect BPH-1 cells was greatly
reduced in Mg260_3 compared to the M. genitalium WT (p &lt; 0.001). This was evident
by infectivity assays and confocal microscopy. Proteomic analysis of BPH-1 cells
infected with either M. genitalium WT
or Mg260_3
%K Cancer
%K ER Stress Proteins
%K Infection
%K &lt
%K i&gt
%K Mycoplasma genitalium&lt
%K /i&gt
%K Prostate
%K Proteomics
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=37479