%0 Journal Article
%T A Novel <i>in Vitro</i> Three-Dimensional Macroporous Scaffolds from Bacterial Cellulose for Culture of  Breast Cancer Cells
%A Guangyao Xiong
%A Honglin Luo
%A Feng Gu
%A Jing Zhang
%A Da Hu
%A Yizao Wan
%J Journal of Biomaterials and Nanobiotechnology
%P 316-326
%@ 2158-7043
%D 2013
%I Scientific Research Publishing
%R 10.4236/jbnb.2013.44040
%X <p class=\"MsoNormal\">
	<span lang=\"EN-US\" style=\"font-family:Verdana;\">In this work, patterned macropores
with a diameter</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\"><span style=\"font-family:Verdana;\">larger
than 100 ¦Ìm were introduced to pristine three-dimensional (3D) nanofibrous
bacterial cellulose (BC) scaffolds by using the infrared laser micromachining </span><span style=\"font-family:Verdana;\">technique in an attempt to create an </span><i><span style=\"font-family:Verdana;\">in vitro</span></i><span style=\"font-family:Verdana;\"> model for the culture of breast cancer cells. The
morphology, pore structure, and mechanical per</span></span><span lang=\"EN-US\" style=\"font-family:Verdana;\">formance of the obtained patterned
macroporous BC (PM-BC) scaffolds were characterized by scanning electron mi</span><span lang=\"EN-US\" style=\"font-family:Verdana;\">croscopy (SEM), mercury intrusion
porosimeter, and mechanical testing. A human breast cancer cell (MDA-MB-231)
line was cultured onto the PM-BC scaffolds to investigate the role of
macropores in the control of cancer cell behavior. MTT assay, SEM, and
hematoxylin and eosin (H&amp;E) staining were employed to determine cell
adhesion, growth, pro</span><span lang=\"EN-US\" style=\"font-family:Verdana;\">liferation,
and infiltration. The PM-BC scaffolds were found to be able to promote cellular
adhesion and proliferation on the scaffolds, and further to allow for cell
infiltration into the PM-BC scaffolds. The results demonstrated that BC scaf</span><span lang=\"EN-US\"><span style=\"font-family:Verdana;\">folds with laser-patterned macropores
were promising for the </span><i><span style=\"font-family:Verdana;\">in vitro</span></i><span style=\"font-family:Verdana;\"> 3D
culture of breast cancer cells.</span></span><span lang=\"EN-US\"><o:p></o:p></span> 
</p>
%K 3D Culture
%K Scaffold
%K Bacterial Cellulose
%K Cancer Cell
%K Macropore
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=36832