%0 Journal Article
%T Characterization of randomly amplified polymorphic DNA (RAPD) fragments revealing clonal variability in cercariae of avian schistosome <i>Trichobilharzia szidati</i> (Trematoda: Schistosomatidae)
%A Anna Korsunenko
%A Galina Chrisanfova
%A Alexander Arifov
%A Alexey Ryskov
%A Seraphima Semyenova
%J Open Journal of Genetics
%P 141-158
%@ 2162-4461
%D 2013
%I Scientific Research Publishing
%R 10.4236/ojgen.2013.33017
%X <p style=\"text-align:justify;\">
	<span style=\"font-size:10.0pt;\"><span style=\"font-family:Verdana;\">Recently we applied randomly amplified
polymorphic DNA (RAPD) fingerprinting to detect clonal variability among
individual cercariae within daughter sporocysts and rediae of 10 digenean trematodes
(Platyhelminthes: Trematoda). The most variable RAPD patterns were obtained
for Schistosomatidae representative-</span><span style=\"font-family:Verdana;\">avian schistosome </span><i><span style=\"font-family:Verdana;\">Trichobilharzia szidati</span></i><span style=\"font-family:Verdana;\">. In this
work, 50 polymorphic DNA fragments of approximately 300-1500 bp from RAPD
patterns of individual </span><i><span style=\"font-family:Verdana;\">T. szidati </span></i><span style=\"font-family:Verdana;\">cercariae
were cloned and sequenced. As a result genomic DNA sequences (total length of
approximately 41,000 bp) revealing clonal variability in </span><i><span style=\"font-family:Verdana;\">T. szidati </span></i><span style=\"font-family:Verdana;\">cercariae were obtained and analyzed. The analysis
indicated that these sequences contained tandem, inverted and dispersed repeats
as well as regions homological to retroelements of two human parasites, </span><i><span style=\"font-family:Verdana;\">Schistosoma mansoni </span></i><span style=\"font-family:Verdana;\">and </span><i><span style=\"font-family:Verdana;\">S. japonicum</span></i><span style=\"font-family:Verdana;\">. Tandem and inverted
repeats constituted 8.9% and 22.1% respectively, while the percentage of dispersed
repeats was 21.0%. The average content of these components was 41.7% with the average
AT content being 59.0%. About 40% of sequences included regions ranging in
length from 96 to 1005 bp which displayed amino acid homology with open reading
frame </span><i><span style=\"font-family:Verdana;\">pol</span></i><span style=\"font-family:Verdana;\"> products of </span><i><span style=\"font-family:Verdana;\">S. mansoni </span></i><span style=\"font-family:Verdana;\">and </span><i><span style=\"font-family:Verdana;\">S. japonicum </span></i><span style=\"font-family:Verdana;\">retroelements: non-long terminal repeat retrotransposons
(nLTRs, 76%), long terminal repeat retrotransposons (LTRs, 14%), and
Penelope-like elements (PLEs, 10%). Most of these regions (86.4%) contained
frameshifts, gaps, and stop-codons. The largest portion of them was homological
to nLTRs of </span></span><span style=\"font-size:10pt;line-height:1.5;font-family:Verdana;\">the RTE clade (67%). The
number of sequences
%K RAPD Variability
%K Cercariae Heterogeneity
%K &lt
%K i&gt
%K Trichobilharzia szidati&lt
%K i&gt
%K Repetitive DNA
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=36327