%0 Journal Article %T Identification of Phenotypic and Genotypic Variability among the Isolates of <i>Ramularia areola</i> of Brazilian Cotton %A Larissa Girotto %A Mariana S. Marangoni %A Janaina N. Matos %A Rafael Galbieri %A Wilson P. Almeida %A Yeshwant R. Mehta %J American Journal of Plant Sciences %P 1893-1898 %@ 2158-2750 %D 2013 %I Scientific Research Publishing %R 10.4236/ajps.2013.49232 %X
Genotypic and
phenotypic variation among 16 isolates of Ramularia
areola of Gossypium hirsutum collected from five different geographical regions of Brazil was studied
through virulence spectrum on three cultivars in the glasshouse and through
ERIC- and REP-PCR and ITS1-5.8S-ITS2 rDNA analysis. Difference in virulence
spectrum and molecular analysis of some isolates was observed. ERIC- and
REP-PCR showed similar results and revealed a high level of diversity among the
isolates. A unique profile for both ERIC and REP was obtained for most
isolates. On the other hand, the ITS rDNA analysis did not show different
PCR-RFLP patterns. While some isolates differed among each other considering
genotypic and phenotypic reactions, no clear cut evidence was found about the
existence of genetic lineages of R.
areola in