%0 Journal Article %T Low-Density Co-Inoculation of Myanmar <i>Bradyrhizobium yuanmingense</i> MAS34 and <i>Streptomyces griseoflavus</i> P4 to Enhance Symbiosis and Seed Yield in Soybean Varieties %A Khin Myat Soe %A Takeo Yamakawa %J American Journal of Plant Sciences %P 1879-1892 %@ 2158-2750 %D 2013 %I Scientific Research Publishing %R 10.4236/ajps.2013.49231 %X
This study examined whether low-density co-inoculation of Myanmar Bradyrhizobium yuanmingense strain MAS34 and Streptomyces griseoflavus P4 would enhance nodulation, N2 fixation, and seed yield in two soybean varieties. A field experiment was conducted during the July to November 2012 growing season at Kyushu University Farm, Japan, using a split-plot design with three replications and the following four treatments: T1, an uninoculated treatment with peat moss (uninoculated); T2, a single inoculation with S. griseoflavus P4 (P4); T3, a single inoculation of B. yuanmingense MAS34 (MAS34); and T4, a dual inoculation of P4 with MAS34 (P4 + MAS34). Two varieties of soybean, Yezin-3 (Rj4) and Yezin-6 (non-Rj), were used. The N2 fixation activity of soybean was evaluated by the relative ureide method using xylem solute from root bled sap at the early pod-fill stage (R3.5). Dry matter production, N2 fixation, and seed yield were significantly (P < 0.01) different between the inoculated treatments. The effect of variety was also sig