%0 Journal Article
%T An LC-MS/MS Method for Determination of Imperatorin and Isoimperatorin in Rat Plasma and Application to a Pharmacokinetic Study
%A Junbo Xing
%A Hong Cao
%A Yumin Chen
%A Caihong Shui
%A Tingting Shan
%A Dan Hu
%J Pharmacology & Pharmacy
%P 417-424
%@ 2157-9431
%D 2013
%I Scientific Research Publishing
%R 10.4236/pp.2013.45059
%X <span style=\"font-family:'Times New Roman','serif';color:black;font-size:10pt;\"><span style=\"font-family:Verdana;\">A highly sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed for the de-termination of imperatorin and isoimperatorin in rat plasma using psoralen as internal standard. Analyses were performed on a Shimadzu HPLC system using a Shimadzu C18 column eluted with a mobile phase of methanol-water (25:75), containing 0.1% formic acid at a flow rate of 0.4 ml/min. The detection was performed by positive ion elec-trospray ionization in multiple reactions monitoring mode, monitoring the transitions </span><i><span style=\"font-family:Verdana;\">m/z </span></i><span style=\"font-family:Verdana;\">203¡ú147 and </span><i><span style=\"font-family:Verdana;\">m/z </span></i><span style=\"font-family:Verdana;\">203¡ú159 for imperatorin and isoimperatorin, respectively. The method was validated over the concentration range of 2.5 -1000.0 ng/mL for imperatorin and isoimperatorin. The limits of detection and quantification were 1.0 and 3.0 ng/mL, repectively for both analytes. The intra-day and inter-day accuracy and precision of the assay were less than 10.9%. This method has been applied successfully to a pharmacokinetic study involving the intragastric administration of extract of </span><i><span style=\"font-family:Verdana;\">Angelicae Dahuricae Radix </span></i><span style=\"font-family:Verdana;\">to rats.</span></span>
%K Imperatorin
%K Isoimperatorin
%K LC-MS/MS
%K Pharmacokinetic
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=35832