%0 Journal Article
%T Angiotensin-(1 - 7) and Human Chorionic Gonadotropin (hCG) Modulate the Nuclear Transcription Factors or Nuclear Receptors Genes in the Tumorigenic Undifferentiated Breast Cancer Cell Line SKBR3
%A Isidoro Binda Neto
%A Samuel Marcos Ribeiro de Noronha
%A Silvana Aparecida Alves Correa de Noronha
%A Maria Del Carmen Garcia Molina Wolgien
%A Alexandre Jesus Barros
%A Clovis Ryiuchi Nakaie
%A Suma Imura Shimuta
%A Gil Facina
%A Ismael Dale Cotrim Guerreiro da Silva
%J Journal of Cancer Therapy
%P 70-74
%@ 2151-1942
%D 2013
%I Scientific Research Publishing
%R 10.4236/jct.2013.47A011
%X <p class=\"MsoNormal\">
	<span lang=\"EN-US\">Breast cancer is the most common
cancer among women. Angiotensin-(1</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">-</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">7) [Ang-(1</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">-</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">7)] has been correlated with cancer
antiproliferative and apoptotic effects, similar properties of the human Chorionic Gonadotrofin (hCG)<i>.</i> The aims of this work are to evaluate the role of Ang-(1</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">-</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">7) and of hCG in modulating the
expression of Nuclear Receptors and Coregulators
related genes in the tumorigenic breast cell line SK-BR3. Three experimental
groups were created: control, hCG and hCG</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">+</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">Ang-(1</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">-</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">7). Cells were treated for 11 days and
then had their RNA extracted. Samples were loaded into PCR Array plates
containing 84 genes relate to Nuclear
Receptors and Coregulators pathways. Gene expression data were used to
construct canonical pathways<i> </i>(Metacore<sup>TM</sup>).
hCG and hCG</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">+</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">Ang-(1</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">-</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">7) treatments markedly modu</span><span lang=\"EN-US\">late the expression
of</span><span lang=\"EN-US\"> Nuclear Receptors and Coregulators<i> </i>related genes. hCG di</span><span lang=\"EN-US\">f</span><span lang=\"EN-US\">ferentially expressed </span><span lang=\"EN-US\">17% of the genes, being 29% upregulated and 71%
downregulated. Meanwhile, hCG</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">+</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">Ang-(1</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">-</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">7) changed the expression of 30% of the genes on the plate, among these
genes 56% were upregulated and 44% downregulated. Among these differentially ex</span><span lang=\"EN-US\">pressed genes, we highlight Esr1, Nr2f2, and Nr2f1, Esr1, Hdac5, and
Nr4A1 (&gt;4 fold). Finally MetaCore analysis based on Gene Ontology (GO)
generated six networks for hCG and ten networks for the
%K Breast Stem Cancer Cells
%K SK-BR3
%K hCG
%K Angiotensin-(1 - 7)
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=35045