%0 Journal Article
%T Transferability and application of microsatellites (SSRs) from <i>Juniperus communis</i> L. to <i>Juniperus procera</i> Hochst. Ex endl.
%A Demissew Sertse
%A Oliver Gailing
%A Nicolas-George Eliades
%A Reiner Finkeldey
%J Open Journal of Genetics
%P 115-126
%@ 2162-4461
%D 2013
%I Scientific Research Publishing
%R 10.4236/ojgen.2013.32015
%X <p>
	<span>Transferability of five nuclear microsatellite </span><span>markers</span><span> (</span><i><span>Jc-</span></i><span>16<i>, Jc-</i>31<i>, Jc-</i>32<i>, Jc-</i>35<i> </i>and<i> Jc-</i>37)</span><span> that were originally developed for <i>J.
communis</i> was tested to <i>J. procera</i>. <i>Jc-</i>31<i> &amp; Jc-</i>37<i> </i>showed
successful amplifications and polymorphism in <i>J. procera.</i> <i>Jc-</i>35 which
had been reported as polymorphic in <i>J.
communis</i> was monomorphic in <i>J.
procera </i>while the primer pair for <i>Jc-</i>32
failed to record any amplification. </span><span>The remaining one primer
pair (<i>Jc-</i>16)<i> </i>showed double loci ampli-fication in both<i> J. procera </i>and the control<i> J. communis </i>suggesting further examination of the primer pair and its
binding sites<i>.</i> Genetic variation of
six Ethiopian <i>J. procera</i> populations: Chilimo, Goba, Menagesha-Suba, Wef-Washa,
Yabelo and Ziquala was assessed based on the two </span><span>polymorphic loci (<i>Jc-</i>31 &amp; <i>Jc-</i>37) </span><span>in 20 - 24 individuals of each
population. From these two loci, a total of 41 alleles could be retrieved. Two
populations that are located south east of the Great Rift Valley together
harboured 75% of private alleles signifying their deviant geo-ecological zones
and suggesting special consideration for conservation. Chilimo, which is at the
western margin of <i>Juniper</i> habitat in
Ethiopian central highlands scored the highest fixation (<i>F<sub>IS</sub> </i>= </span><span>0.584)</span><span> entailing lower immigrant
genes and hence higher inbreeding. The <i>AMOVA</i> revealed that 97% of the variation resided within the&nbsp;population while still among
population variation was significant (p &lt; 0.05).</span><b><span style=\"font-size:10.0pt;\"></span></b> 
</p>
%K Ethiopia
%K &lt
%K i&gt
%K Juniperus communis&lt
%K /i&gt
%K Microsatellites
%K Transferability
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=33619