%0 Journal Article
%T Isolation and characterization of polymorphic microsatellite loci for the valuable medicinal plant <i>Astragalus mongholicus</i>
%A Ao Wang
%A Wujisguleng Wujisguleng
%A Yujing Liu
%A Yue Liu
%A Chunlin Long
%J Open Journal of Genetics
%P 89-92
%@ 2162-4461
%D 2013
%I Scientific Research Publishing
%R 10.4236/ojgen.2013.32011
%X <p>
	<i><span style=\"font-size:10.0pt;\">Astragalus
mongholicus</span></i><span style=\"font-size:10.0pt;\"> (Fabaceae) is a perennial herb and
a widely used medicinal plant in traditional Chinese medicine (TCM) known as
Radix Astragali (<a name=\"OLE_LINK14\"></a><a name=\"OLE_LINK13\"></a><i>Huangqi</i>).
It was reported to have hepatoprotective, cardioprotective, antidiabetic, antiaging,
sedative and immunopotentiating e</span><span style=\"font-size:10.0pt;\">ff</span><span style=\"font-size:10.0pt;\">ects, and could also be used
as an adjuvant medicine during cancer therapy. Until now, there is only a
little research on its population genetics and no report on development of
microsatellite loci for this plant. In this study, a microsatellite-enriched
genomic DNA library of <i>A. mongholicus</i> was developed and screened to identify marker loci. Ten polymorphic loci were
isolated and analyzed by screening 30 individuals. The number of alleles per locus
ranged from 4 to 19, with an average of 12.3 alleles per locus. The observed
heterozygosity (<i>H</i><sub>O</sub>) and
the expected heterozygosity (<i>H</i><sub>E</sub>)
ranged from 0.367 to 1.000 and from 0.395 to 0.912, respectively. The
polymorphism information content (PIC) varied from 0.361 to 0.888, with an
average of 0.762. This is the first report on characterization of microsatellite
loci for <i>A. mongholicus</i>, and these
markers will be useful for population genetics and molecular ecology studies of
this plant.</span> 
</p>
%K &lt
%K i&gt
%K Astragalus mongholicus&lt
%K /i&gt
%K Medicinal Plant
%K Microsatellite
%K Molecular Marker
%K Population Genetics
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=33610