%0 Journal Article
%T A New Metal Tag for Highly Selective and Sensitive Analyses of Amino Acids and Dipeptides by HPLC/ICP-MS
%A Daigo Iwahata
%A Kazuki Nakamura
%A Rie Yamada
%A Hiroshi Miyano
%A Naoyuki Yamada
%J Journal of Analytical Sciences, Methods and Instrumentation
%P 80-89
%@ 2164-2753
%D 2013
%I Scientific Research Publishing
%R 10.4236/jasmi.2013.32010
%X <p align=\"justify\">
	<span style=\"font-family:Verdana;\">We have developed a novel metal tag, bis(ethylenediamine)-4</span><span style=\"font-family:Verdana;\">'</span><span style=\"font-family:Verdana;\">-methyl-4-carboxybipyridine-ruthenium N-succinimidyl ester (ECRS) for sensitive analysis of amino acids using high performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICP-MS). ECRS is a functional reagent, containing an ester group at one end that can be activated to bind to amino group and a chelated ruthenium at the other. The activated ester was reacted briefly with amino groups under weakly alkaline conditions. The ruthenium was detected sensitively by ICP-MS. ECRS was reacted with 17 proteinogenic amino acids in borate buffer. The derivatives were separated by reversed phase HPLC and identified by quadrupole-based ICP-MS. ECRS was suitable for speciation; low molecular weight compounds containing amino groups. We have thus established a quantitative analytical method for amino acids and dipeptides. The detection limits of branched amino acids (signal-to-noise ratio of 3) were 1.5 nmol</span><span style=\"font-family:Verdana;\">กค</span><span><span>L<sup>-</sup></span><span style=\"font-family:Verdana;\"><sup>1</sup></span></span><span><span><sup></sup></span><span style=\"font-family:;\"><sup></sup></span><span><sup><span style=\"font-family:Verdana;\"> </span></sup><span style=\"font-family:Verdana;\">in the standard solution (100 amol per injection)</span></span></span><span style=\"font-family:Verdana;\">. </span> 
</p>
%K Metal Tag
%K HPLC/ICP-MS
%K Amino Acid
%K Dipeptide
%K Derivatization
%K Speciation
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=32357