%0 Journal Article
%T Expression of Large Conductance, Voltage- and Ca<sup>2+</sup>-Activated K<sup>+</sup> (BK) Channels in Human Urinary Bladder: Alteration of Subunit Expression Profile in Association with Bladder Outlet Obstruction
%A Hidenori Zakoji
%A Hideki Kobayashi
%A Mitsuharu Yoshiyama
%A Masayuki Takeda
%A Isao Araki
%J Open Journal of Urology
%P 47-52
%@ 2160-5629
%D 2013
%I Scientific Research Publishing
%R 10.4236/oju.2013.32009
%X <p class=\"MsoNormal\" style=\"text-align:justify;\">
	<b><span lang=\"EN-US\">Purpose:</span></b><span lang=\"EN-US\"> Large conductance, voltage- and Ca<sup>2+</sup>-activated K<sup>+</sup> (BK) channel is thought to have a central role to regulate urinary bladder
smooth muscle functions, and its dysfunction may lead to increase of urination
frequency and overac</span><span lang=\"EN-US\">tive bladder. The present study aims to investigate the
expression pattern of BK channel subunits in the human urinary bladder, and how
it changes in association with bladder outlet obstruction (BOO). <b>Materials and Methods:</b> Human bladders
were obtained from 7 controls without prostatic enlargement and lower urinary
tract symptoms and 4 BPH patients with clinically diagnosed overactive bladder
who were verified by the International Prostate Symptom Score (IPSS) and
prostate volume. The expression and location of BK channel protein complex was
examined using immuno</span><span lang=\"EN-US\">histochemistry with affinity-purified anti-BK<i>¦Á</i> antibodies. A real-time RT-PCR was
used to quantify the expression of each BK channel subunit (<i>¦Á</i> and <i>¦Â</i>1</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">-</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">4) gene in the mucosal and muscle layers of human urinary bladder. <b>Results:</b> Im</span><span lang=\"EN-US\">munohistochemical
staining for BK-<i>¦Á</i> protein complex was
localized in the muscle and submucosal regions of urinary bladder. RT-PCR
analysis revealed the presence of <i>¦Á</i>-, <i>¦Â</i>1-, and <i>¦Â</i>4-subunit genes of BK channel in the mucosal layer, <i>¦Á</i>- and <i>¦Â</i>1-subunit in the muscle layer. The expressions of <i>¦Á</i>- and <i>¦Â</i>1-subunit genes in the muscle (<i>¦Á</i>:
p = 0.0003, <i>¦Â</i>1: p = 0.0003) and
mucosal (<i>¦Á</i>: p = 0.03, <i>¦Â</i>1: p = 0.02) layers significantly
decreased in BOO bladders compared with controls. The expression levels of <i>¦Á</i>- and <i>¦Â</i>1-subunit in mucosal layer were statistically correlated with storage
score of IPSS (<i>¦Á</i>; r</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">=</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">0.84, p</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">=</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">0.002, <i>¦Â</i>1; r</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">=</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">0.84, p</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">=</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">0.002),
and
%K BK Channel
%K Overactive Bladder
%K Bladder Outlet Obstruction
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=31316