%0 Journal Article
%T <i>At</i>L1 a Non-LTR Retrotrasposon Fragment in the Genome of <i>Arabidopsis thaliana </i> with Homology to Plants and Animals
%A Giovanna Visioli
%A Elena Maestri
%A Eugenia Polverini
%A Angelo Pavesi
%A Nelson Marmiroli
%J American Journal of Plant Sciences
%P 806-816
%@ 2158-2750
%D 2013
%I Scientific Research Publishing
%R 10.4236/ajps.2013.44099
%X <p class=\"MsoNormal\">
	<span lang=\"EN-GB\"><span style=\"font-family:Verdana;\">We report the isolation of </span><i><span style=\"font-family:Verdana;\">At</span></i><span style=\"font-family:Verdana;\">L1,
a 249 bp non-LTR retrotransposon fragment from </span><i><span style=\"font-family:Verdana;\">Arabidopsis thaliana</span></i><span style=\"font-family:Verdana;\"> by fingerprint</span></span><span lang=\"EN-GB\"><span style=\"font-family:Verdana;\">ing mRNAs extracted from </span><i><span style=\"font-family:Verdana;\">A. thaliana</span></i><span style=\"font-family:Verdana;\"> plants, ecotype Columbia, in different heat stress
conditions. Southern blot and PCR analysis suggested that </span><i><span style=\"font-family:Verdana;\">At</span></i><span style=\"font-family:Verdana;\">L1 occurs as a single- or low-copy insert in the genome of </span><i><span style=\"font-family:Verdana;\">A. thaliana</span></i><span style=\"font-family:Verdana;\"> ecotype Columbia. The
presence of </span><i><span style=\"font-family:Verdana;\">At</span></i><span style=\"font-family:Verdana;\">L</span><st1:chmetcnv unitname=\"in\" sourcevalue=\"1\" hasspace=\"True\" negative=\"False\" numbertype=\"1\" tcsc=\"0\" w:st=\"on\"><span style=\"font-family:Verdana;\">1 in</span></st1:chmetcnv><span style=\"font-family:Verdana;\"> the genome of different </span><i><span style=\"font-family:Verdana;\">Arabidopsis</span></i><span style=\"font-family:Verdana;\"> ecotypes was confirmed by
PCR amplification and se</span></span><span lang=\"EN-GB\"><span style=\"font-family:Verdana;\">quencing
thus excluding all possible contamination. A preliminary scan of the </span><i><span style=\"font-family:Verdana;\">At</span></i><span style=\"font-family:Verdana;\">L1 nucleotide sequence against the
EMBL and NCBI databases revealed a high degree of similarity to a group of LINE
type L1 retrotransposons of mam</span></span><span lang=\"EN-GB\"><span style=\"font-family:Verdana;\">mals
and with a cDNA sequence of </span><i><span style=\"font-family:Verdana;\">Artemisia
annua</span></i><span style=\"font-family:Verdana;\">. A phylogenetic analysis of LINE elements from animals and plants
placed </span><i><span style=\"font-family:Verdana;\">At</span></i><span style=\"font-family:Verdana;\">L1 and </span><i><span style=\"font-family:Verdana;\">A. annua</span></i><span style=\"font-family:Verdana;\"> sequences in close proximity to some mammalian sequences
but distant from the other plants LINE elements including those from </span><i><span
%K Arabidopsis
%K Heat Shock
%K Plant Retrotransposons
%K LINE Retrotransposons
%K Horizontal Transposon Transfer (HTT)
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=29824