%0 Journal Article
%T Efficacy of Antimicrobial Lauric Arginate against <i>Listeria monocytogenes</i> on Stainless Steel Coupons
%A Jasdeep K. Saini
%A Miguel A. Barrios
%A James L. Marsden
%A Kelly J. K. Getty
%A Daniel Y. C. Fung
%J Advances in Microbiology
%P 65-68
%@ 2165-3410
%D 2013
%I Scientific Research Publishing
%R 10.4236/aim.2013.31010
%X <p class=\"MsoNormal\">
	<span lang=\"EN-US\">Commercially processed foods become contaminated with <i>Listeria monocytogenes </i>in
post-processing environments where favorable conditions help the bacteria
thrive. The US Food and Drug Administration has approved Lauric argi</span><span lang=\"EN-US\">nate
(LAE) as generally recognized as safe (GRAS) for certain food applications.
This study evaluated the efficacy of Mirenat-N (LAE dissolved in food-grade
propylene glycol) against <i>L.
monocytogenes</i> on food contact surfaces. A three-strain cocktail of <i>L. monocytogenes </i>was used to inoculate
24 polished stainless steel coupons with three treat</span><span lang=\"EN-US\">ments, 100 ppm and
200 ppm solutions of LAE and water (control); two sub-treatments of high (6 log
CFU/ml) and low (4 log CFU/ml) inoculum levels; and two contact times of 5 and
15 min. Attached bacteria were dislodged by vortexing coupons for 1 min with <st1:chmetcnv w:st=\"on\" tcsc=\"0\" numbertype=\"1\" negative=\"False\" hasspace=\"True\" sourcevalue=\"20\" unitname=\"g\">20 g</st1:chmetcnv> of 3-mm solid glass beads in 10 ml of
0.1% peptone diluent, and bacterial populations were calculated by plating onto
modified oxford medium (MOX) and thin agar layer MOX (TALMOX). The 100</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">ppm
treatment showed average reductions of 1.38 and 2.57 log CFU/coupon at the low
inoculum level and 0.37 and 0.62 log <span style=\"letter-spacing:-0.05pt;\">CFU/coupon
at high inoculum levels, after 5 and 15 min exposure, respectively. For 200 ppm
at the high inoculum level, </span>1.23 and 1.88 log CFU/coupon reductions were
seen for 5 and 15 min, respectively; the low inoculum level at 5 and 15 min
exposure showed reductions of ¡Ü1.5 log CFU/coupon. The 100</span><span lang=\"EN-US\"> </span><span lang=\"EN-US\">ppm LAE
treatment was more effective at low in</span><span lang=\"EN-US\">oculum levels for 5 and 15 min contact times
and may be used to control low levels of contamination of <i>L. monocyto</i></span><i><span lang=\"EN-US\">genes</span></i><span lang=\"EN-US\"> on food contact surfaces.</span><span lang=\"EN-US\"><o:p></o:p></span> 
</p>
%K Lauric Arginate
%K &lt
%K i&gt
%K Listeria monocytogenes&lt
%K /i&gt
%K Stainless Steel Coupons
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=29199