%0 Journal Article %T Use of markers to determine the ileal digestibility of amino acids for swine Uso de indicadores para determinar a digestibilidade ileal de amino¨¢cidos para su¨ªnos %A Paulo Cesar Pozza %A Paulo Cezar Gomes %A Magali Soares Santos Pozza %A Ricardo Vianna Nunes %J Ci¨ºncia e Agrotecnologia %D 2013 %I Universidade Federal de Lavras %X Many markers can be used in digestibility assays for swine, but needs to be evaluated before its use. An assay was carried out with the objective of measuring the coefficients of ileal apparent (CIAD) and standardized digestibility (CISD) of amino acids in meat and bone meal for swine using different markers. Eight crossbred barrows were equipped with T-cannulas in the distal ileum. Four animals received a free protein diet and another four received a diet containing meat and bone meal as the only protein source in the diet. The treatments consisted in evaluating the chromic oxide, acid-insoluble ash naturally occurring in the diet, cellulose and lignin as markers to determine the apparent and standardized digestibility of the amino acids contained in the meat and bone meal. All data were submitted to analysis of variance and means were compared using the Tukey test, with a significance of 5%. The CIAD, obtained using chromic oxide as external marker, were lower (P<0.05) than those obtained when acid insoluble ash, lignin and cellulose were used as markers, observing differences for all the amino acids evaluated. Similarly, the CISD from all the studied amino acids were lower (P<0.05) when the chromic oxide was used for determining the indigestibility factor. It is concluded that chromic oxide used as a external marker, to determine the coefficients of apparent and standardized ileal digestibility of the amino acids of the meat and bone meal, is most appropriate than the acid insoluble ash naturally occurring in diets, lignin and cellulose. Muitos indicadores podem ser utilizados em ensaios de digestibilidade para su¨ªnos, mas precisam ser avaliados antes de serem utilizados. O experimento foi realizado com o objetivo de determinar os coeficientes de digestibilidade ileal aparentes (CDIA) e estandardizados (CDIE) dos amino¨¢cidos da farinha de carne e ossos, para su¨ªnos, utilizando diferentes indicadores. Oito su¨ªnos, machos castrados, foram submetidos ¨¤ cirurgia para aimplanta o de canulas T simples, no ¨ªleo distal. Quatro animais receberam uma dieta isenta de prote¨ªna e outros quatro uma dieta contendo farinha de carne e ossos como ¨²nica fonte prot¨¦ica na dieta. Os tratamentos consistiram em avaliar o ¨®xido cr¨®mico, cinza insol¨²vel em ¨¢cido naturalmente contido na dieta, celulose e lignina como indicadores para determinar os CDIA e CDIE dos amino¨¢cidos da farinha de carne e ossos. Os dados foram submetidos a an¨¢lise de variancia e as m¨¦dias foram comparadas pelo teste de Tukey, ao n¨ªvel de 5% de significancia. Os CDIA, obtidos usando o ¨®xido cr mico co %K Cinza insol¨²vel em ¨¢cido %K celulose %K ¨®xido cr mico %K lignina %K canula T simples %K Acid-insoluble ash %K cellulose %K chromic oxide %K lignin %K simple T-cannula %U http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000300009