%0 Journal Article
%T Study of the correlation between blood cholinesterases activity, urinary dialkyl phosphates, and the frequency of micronucleated polychromatic erythrocytes in rats exposed to disulfoton
%A Mariane Gon£¿alves Santos
%A Ricardo Vilela Vitor
%A Maur¨ªcio Gustavo Nakamura
%A Luana de Souza Morelini
%J Brazilian Journal of Pharmaceutical Sciences
%D 2013
%I Universidade de S?o Paulo
%X Organophosphates (OPs) are widely used as pesticides, and its urinary metabolites as well as the blood cholinesterases (ChEs) activity have been reported as possible biomarkers for the assessment of this pesticide exposure. Moreover, the OPs can induce mutagenesis, and the bone marrow micronucleus test is an efficient way to assess this chromosomal damage. This paper reports a study carried out to verify the correlation among the disulfoton exposure, blood ChEs activity, urinary diethyl thiophosphate (DETP), and diethyl dithiophosphate (DEDTP), as well as micronucleated polychromatic erythrocytes (MNPCEs) frequency. Four groups of rats (n=12) were exposed to disulfoton at 0, 2.8, 4.7, and 6.6 mg kg-1 body weight. The blood ChEs activity, urinary DETP and DEDTP concentrations, and MNPCEs frequency were determined. It was observed that the plasmatic and erythrocytary ChEs activity decreased from 2.9% to 0.5% and from 35.9 to 3.3%, respectively, when the disulfoton dose was increased from 0 to 6.6 mg kg-1 (correlation of 0.99). Urinary DETP and DEDTP concentrations, as well as the MNPCEs frequency, increased from 0 to 6.58 ¦Ìg mL-1, from 0 to 0.04 ¦Ìg mL-1, and from 0 to 1.4%, respectively, when the disulfoton dose was increased from 0 to 6.58 mg kg-1 body weight. Os organofosforados (OPs) s o amplamente usados como praguicidas e a atividade da colinesterase sangu¨ªnea bem como os metab¨®litos urin¨¢rios desses praguicidas t¨ºm sido reportados como biomarcadores eficazes para avaliar casos de exposi  o. Al¨¦m disso, os OPs podem induzir mutag¨ºnese e o teste de micron¨²cleo de medula ¨®ssea ¨¦ uma boa alternativa para avaliar os danos cromoss micos. Esse artigo reporta um estudo sobre a correla  o entre a exposi  o a dissulfoton, a atividade da colinesterase sangu¨ªnea, a excre  o urin¨¢ria de dietil tiofosfato e dietil ditiofosfato e a frequ¨ºncia de micron¨²cleos em eritr¨®citos policrom¨¢ticos. Quatro grupos de ratos (n=12) foram expostos a dissulfotom nas doses de 0, 2,8, 4,7, e 6,6 mg kg-1 de peso corp¨®reo. A atividade da colinesterase sangu¨ªnea as concentra  es urin¨¢rias de dietil tiofosfato e dietil ditiofosfato e a frequ¨ºncia de micron¨²cleos foram determinadas. Os resultados demonstraram que as atividades da colinesterase plasm¨¢tica e eritrocit¨¢ria diminu¨ªram de 2,9 para 0,5% e de 35,9 para 3,3% , respectivamente, quando a dose de dissulfoton foi aumentada de 0 para 6,6 mg kg-1 (correla  o de 0,99). As concentra  es urin¨¢rias de dietil tiofosfato e dietil ditiofosfato bem como a frequ¨ºncia de micron¨²cleos aumentaram de 0 a 6,56 ¦Ìg mL-1, 0 a 0.04 ¦Ìg mL-1 e de 0 a 1.
%K Pesticidas
%K Organofosforados
%K Dissulfoton
%K Dialquil fosfatos
%K Colinesterase sangu¨ªnea
%K Eritr¨®citos policrom¨¢ticos micronucleados
%K Pesticides
%K Organophosphate
%K Disulfoton
%K Dialkyl phosphate
%K Blood cholinesterase
%K Micronucleated polychromatic erythrocytes
%U http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100016