%0 Journal Article
%T Anti-inflammatory and antinociceptive activities of methanolic extract from red seaweed Dichotomaria obtusata
%A Neivys Garc¨ªa Delgado
%A Ana Iris Fr¨ªas V¨¢zquez
%A Hiran Cabrera S¨¢nchez
%A Roberto Men¨¦ndez Soto del Valle
%J Brazilian Journal of Pharmaceutical Sciences
%D 2013
%I Universidade de S?o Paulo
%X The aim of the present work was to investigate the anti-inflammatory and antinociceptive effects of methanolic extract from D. obtusata using classic models in mice (croton oil-induced ear edema and acetic acid-induced writhing) and a phospholipase A2 activity test. Qualitative analysis of the chemical composition of seaweed was also determined by extraction with solvents of increasing polarity and precipitation and color tests. Results of qualitative chemical study showed the presence of lactonic and phenolic compounds, reduced carbohydrates, other sugars, flavonoids, fatty compounds, triterpenes and steroids. The extract inhibited mouse ear edema in a dose-dependent manner with an efficacy higher than 90% and a mean effective dose of 4.87¦Ìg/ear, while intraperitoneal administration presented a moderate activity. The extract did not inhibit phospholipase A2 activity. In the writhing test, the intraperitoneal administration of the extract showed a strong antinociceptive activity (80.2%), while the oral route showed a lower efficacy. In conclusion, this study demonstrated the anti-inflammatory and antinociceptive effects of methanol extract of D. obtusata in experimental models, suggesting its therapeutic potential in the treatment of peripheral painful and/or inflammatory pathologies. O objetivo do presente trabalho foi investigar os efeitos antiinflamat¨®rios e antinociceptivos de um extrato metan¨®lico de D. obtusata, utilizando modelos cl¨¢ssicos em ratos (teste do edema de orelha induzido por ¨®leo de cr¨®ton e teste de contor  es induzidas por ¨¢cido ac¨¦tico) e um teste de atividade de fosfolipase A2. A an¨¢lise qualitativa da composi  o qu¨ªmica das algas foi tamb¨¦m determinada atrav¨¦s de extra  o com solventes de polaridade crescente e testes de precipita  o e cor. Os resultados do estudo de qu¨ªmica qualitativa mostraram a presen a de compostos lact nicos e fen¨®licos, hidratos de carbono reduzidos e outros a ¨²cares, flavonoides, compostos graxos, triterpenos e esteroides. O extrato inibiu o edema de orelha dos ratos de um modo dependente da dose com efic¨¢cia superior a 90% e dose m¨¦dia efetiva de 4.87¦Ìg/orelha, enquanto a administra  o intraperitoneal apresentou atividade moderada. O extrato n o inibiu a atividade da fosfolipase A2. No teste de contor  o, a administra  o intraperitoneal do extrato mostrou forte atividade antinociceptiva (80,2%), enquanto a administra  o oral mostrou menor efic¨¢cia. Em conclus o, este estudo demonstrou os efeitos antiinflamat¨®rios e antinociceptivos do extrato metan¨®lico de D. obtusata em modelos experimentais, sugerindo
%K Dichotomaria obtusata
%K Dichotomaria obtusata
%K Dichotomaria obtusata
%K Teste do edema de orelha
%K Teste de contor  es
%K Dichotomaria obtusata
%K Dichotomaria obtusata
%K Dichotomaria obtusata
%K Mouse ear edema test
%K Writhing test
%U http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100008