%0 Journal Article
%T Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata Establecimiento y optimizaci車n de marcadores moleculares ISSR y SAMPL como una herramienta para programas de mejoramiento de Pinus radiata
%A Priscila Moraga-Suazo
%A Rodrigo Hasb迆n
%A Claudio Balocchi
%A Sof赤a Valenzuela
%J Bosque (Valdivia)
%D 2012
%I Universidad Austral de Chile
%X Pinus radiata is a conifer of recognized economic value. Studies at genetic and molecular level to support breeding programs of radiata pine have been conducted with difficulty due to its large and complex genome. The development of molecular markers has allowed performing studies on genetic variation, identification of clones and construction of linkage maps. In order to increase the genetic molecular markers in P. radiata, two inter simple sequence repeat (ISSR) markers and twelve selective amplifications of microsatellite polymorphic loci (SAMPL) primer combinations were tested, using a first-generation full-sib family of 86 individuals. Polymerase chain reaction (PCR) products were visualized by capillary electrophoresis and polymorphism was detected by presence or absence of a particular fragment in one P. radiata parental. A total of 18 polymorphic fragments were found for two ISSR primers tested, with an average segregation distortion of 33 %. SAMPL markers yielded 85 polymorphic fragments with eight primer combinations, with an average of 22.3 % segregation distortion. Both methodologies showed a good reproducibility, with easy implementation and useful for genetic analysis on P. radiata. Pinus radiata es una con赤fera de reconocido valor econ車mico, en la cual los estudios a nivel gen谷tico y molecular, han sido conducidos con dificultad en programas de mejoramiento debido a su genoma complejo y de gran tama o. El desarrollo de marcadores moleculares ha permitido realizar estudios de variaci車n gen谷tica, validar clones y construir mapas de ligamiento. Para lograr incrementar la disponibilidad de marcadores moleculares en P. radiata, se examinaron dos marcadores ISSR (inter simple sequence repeat) y 12 combinaciones de partidores SAMPL (selective amplification of microsatellite polymorphic loci), usando una familia F1 de 86 hermanos completos de primera generaci車n. Los productos de PCR (polymerase chain reaction) se visualizaron mediante electroforesis capilar y la selecci車n de fragmentos polim車rficos se efectu車 a base de la presencia o ausencia de un fragmento en uno de los parentales de la familia de P. radiata. Un total de 18 fragmentos polim車rficos fueron encontrados para los dos partidores ISSR, con una distorsi車n de segregaci車n promedio de 33 %. Los marcadores SAMPL generaron 85 fragmentos polim車rfcos empleando ocho combinaciones, con un promedio de 22,3 % de distorsi車n de segregaci車n. Ambas metodolog赤as mostraron buena reproducibilidad, f芍cil implementaci車n y utilidad para an芍lisis gen谷tico en P. radiata.
%K electroforesis capilar
%K distorsi車n de segregaci車n
%K especies forestales
%K capillary electrophoresis
%K segregation distortion
%K forest trees
%U http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-92002012000100011