%0 Journal Article
%T Relationship between Duffy blood groups genotypes and malaria infection in different ethnic groups of Choco- Colombia
%A Gonzalez
%A Lina
%A Vega
%A Jorge
%A Ramirez
%A Jose L
%A Bedoya
%A Gabriel
%J Colombia M¨¦dica
%D 2012
%I Universidad del Valle
%X Background: The negative homozygous condition for the Duffy blood group (Fy-/Fy-) confers natural resistance to Plasmodium vivax infection. In this direction, studies carried out in Colombia are scarce.Objective: To describe the relationship between Duffy genotypes in three ethnic communities in La Italia (Choc¨®) and malaria infection.Methodology: a descriptive, cross-sectional study in symptomatic and asymptomatic malaria subjects. Sample size : AfroAmerican, 73; Amerindian (Ember¨˘), 74 and Mestizo, 171. Presence of Plasmodium infection was assessed by thick smear and the status of the Duffy gene by PCR and RFLP in order to identify the substitutions T-46C y A131G which origin the genotypes T/T, T/C , C/C y G/G, G/A, A/A.Results: Infection by Plasmodium was detected in 17% with 62% due to P. falciparum and 27% to P. vivax. Duffy genotypes were significantly associated to ethnicity (p=0,003). Individuals with the C/C, A/A diplotype were exclusively infected by P. falciparum, whereas other diplotypes were infected with either species. In the Amerindian and Mestizo populations, the frequency of the T-46 allele was 0,90-1,00, among Afrocolombians this was 0,50, equal to the C allele and with absence of heterozygous At locus 131, the highest frequency of the G allele was 0,30 in Amerindians and the A allele was 0,69 in Afrocolombians. Conclusions: In the Amerindian and mestizo populations studied, a predominance of the allele T-46 (FY+) was observed, but P. vivax was not the most common. Infection by P. vivax was out ruled in all FY- individuals.
%K Duffy Blood-Group System
%K ethnic groups
%K malaria
%K Plasmodium falciparum
%K Plasmodium vivax
%K Genotypes
%K PCR-RFLP
%K Colombia.
%U http://colombiamedica.univalle.edu.co/index.php/comedica/article/view/933/1652