%0 Journal Article
%T Taxonomic considerations among and within some Egyptian taxa of Capparis and related genera (Capparaceae) as revealed by RAPD fingerprinting
%A Moubasher
%A H.
%A Abd El-Ghani
%A M. M.
%A Kamel
%A W.
%A Mansi
%A M.
%J Collectanea Botanica
%D 2011
%I Consejo Superior de Investigaciones Cient¨ªficas
%X Taxonomic considerations among and within some Egyptian taxa of Capparis and related genera (Capparaceae) as revealed by RAPD fingerprinting.- This investigation was carried out to assess the taxonomic relationships among eight taxa of the Egyptian members of Capparaceae based on random amplified polymorphic DNA markers, and to compare the results with those obtained from morphological studies. A total of 46 bands were scored for three RAPD primers corresponding to an average of 15.3 bands per primer. The three primers (A03, A07 and A09) revealed eight polymorphic RAPD markers among the studied taxa ranging in size from 200 bp to 1000 bp. Jaccard¡¯s coefficient of similarity varied from 0.28 to 0.84, indicative of high level of genetic variation among the genotypes studied. UPGMA cluster analysis indicated three distinct clusters, one comprised Cleome amblyocarpa and Gynandropsis gynandra, while another included two clusters at 0.74 phenon line; one for Capparis decidua, and the other for Capparis sinaica and all varieties of Capparis spinosa. The four varieties of Capparis spinosa were segregated at 0.84 phenon line. However, one of these varieties was more closely related to Capparis sinaica than to the other three varieties of C. spinosa. The RAPD analysis reported here confirms previous studies based on morphological markers. Consideraciones taxon¨®micas sobre algunos taxones egipcios de Capparis y g¨¦neros relacionados (Capparaceae) a partir de RAPDs.- El objetivo de este trabajo es investigar las relaciones taxon¨®micas entre ocho taxones pertenecientes a las Capparaceae en base a marcadores de tipo RAPD, y comparar los resultados con los obtenidos previamente en estudios morfol¨®gicos. Se han contabilizado un total de 46 bandas para tres pares de cebadores, con una media de 15,3 bandas por cebador. Los tres pares de cebadores (A03, A07 y A09) revelan ocho marcadores polim¨®rficos entre los taxones estudiados, de entre 200 y 1000 pares de bases. El coeficiente de similaridad de Jaccard var¨ªa entre 0,28 y 0,84, indicativo de un alto nivel de variaci¨®n gen¨¦tica entre los genotipos estudiados. El an¨¢lisis UPGMA muestra tres grupos distintos, el primero comprende Cleome amblyocarpa y Gynandropsis gynandra, mientras que el segundo incluye dos grupos a la altura del valor 0,74 del dendrograma: uno se corresponde con Capparis decidua, y el otro comprende Capparis sinaica y todas las variedades de Capparis spinosa. Las cuatro variedades de C. spinosa se segregan a la altura del valor 0,84 del dendrograma. Sin embargo, una de estas variedades est¨¢ m¨¢s relacionad
%K Capparis
%K Cleome
%K Cluster analysis
%K Egypt
%K Genetic relationships
%K Gynandropsis
%K RAPD-PCR
%K Taxonomy
%K UPGMA
%K Capparis
%K Cleome
%K an¨¢lisis cluster
%K Egipto
%K Gynandropsis
%K RAPD-PCR
%K relaciones gen¨¦ticas
%K taxonom¨ªa
%K UPGMA
%U http://collectaneabotanica.revistas.csic.es/index.php/collectaneabotanica/article/view/182/184