%0 Journal Article
%T Karyotype analysis of four jewel-beetle species (Coleoptera, Buprestidae) detected by standard staining, C-banding, AgNOR-banding and CMA3/DAPI staining
%A Gayane Karagyan
%A Dorota Lachowska
%A Mark Kalashian
%J Comparative Cytogenetics
%D 2012
%I Pensoft Publishers
%R 10.3897/compcytogen.v6i2.2950
%X The male karyotypes of Acmaeodera pilosellae persica Mannerheim, 1837 with 2n=20 (18+neoXY), Sphenoptera scovitzii Faldermann, 1835 (2n=38¨C46), Dicerca aenea validiuscula Semenov, 1895 ¨C 2n=20 (18+Xyp) and Sphaerobothris aghababiani Volkovitsh et Kalashian, 1998 ¨C 2n=16 (14+Xyp) were studied using conventional staining and different chromosome banding techniques: C-banding, AgNOR-banding, as well as fluorochrome Chromomycin A3 (CMA3) and DAPI. It is shown that C-positive segments are weakly visible in all four species which indicates a small amount of constitutive heterochromatin (CH). There were no signals after DAPI staining and some positive signals were discovered using CMA3 staining demonstrating absence of AT-rich DNA and presence of GC-rich clusters of CH. Nucleolus organizing regions (NORs) were revealed using Ag-NOR technique; argentophilic material mostly coincides with positive signals obtained using CMA3 staining.
%K Coleoptera
%K Buprestidae
%K karyotypes
%K Ag-banding
%K C-banding
%K CMA3-staining
%K DAPI-staining
%U http://www.pensoft.net/journals/compcytogen/article/2950/