%0 Journal Article
%T LC-MS metabolomics from study design to data-analysis ¨C using a versatile pathogen as a test case
%A Maya Berg
%A Manu Vanaerschot
%A Andris Jankevics
%A Bart Cuypers
%J Computational and Structural Biotechnology Journal
%D 2013
%I Research Network of Computational and Structural Biotechnology
%X Thanks to significant improvements in LC-MS technology, metabolomics is increasingly used as a tool to discriminate the responses of organisms to various stimuli or drugs. In this minireview we discuss all aspects of the LC-MS metabolomics pipeline, using a complex and versatile model organism, Leishmania donovani, as an illustrative example. The benefits of a hyphenated mass spectrometry platform and a detailed overview of the entire experimental pipeline from sampling, sample storage and sample list set-up to LC-MS measurements and the generation of meaningful results with state-of-the-art data-analysis software will be thoroughly discussed. Finally, we also highlight important pitfalls in the processing of LC-MS data and comment on the benefits of implementing metabolomics in a systems biology approach.
%K mass spectrometry
%K HILIC
%K Leishmania
%K unicellular trypanosomatid parasites
%K global molecular profiles
%K systems biology
%U http://journals.sfu.ca/rncsb/index.php/csbj/article/view/csbj.201301002