%0 Journal Article
%T Backbone NMR assignment of a hypothetical protein MJ0754 from Methanococcus jannaschii DSM 2661
%A Eun Hye Lee
%A Kyoung-Seok Ryu
%A Kyungmin Kim
%A Young Ho Jeon
%J Journal of Analytical Science & Technology
%D 2011
%I 
%X MJ0754 is an unknown hypothetical protein from hyperthermophilic archaeon Methanococcus jannaschii DSM 2661. Here we report the protein purification and NMR spectroscopic study of the N-terminal deleted truncated form of recombinant MJ0754 protein (¦¤10-MJ0754). We aquired 3D NMR spectra and assigned all the backbone chemical shifts including C¦Á, C¦Â, CO, HN, and N of ¦¤10-MJ0754. The secondary structure of ¦¤10-MJ0754 was estimated from the assigned backbone chemical shifts. This NMR result is very useful for further structural and functional study of MJ0754 protein.
%K MJ0754
%K Methanococcus jannaschii
%K backbone assignment
%K NMR
%U http://www.jastmag.org/journal/view.php?number=35