%0 Journal Article
%T Preparation and characterization of two types of covalently immobilized amyloglucosidase
%A NENAD MILOSAVIC
%A RADIVOJE PRODANOVIC
%A SLOBODAN JOVANOVIC
%A IRENA NOVAKOVIC
%J Journal of the Serbian Chemical Society
%D 2005
%I Serbian Chemical Society
%X Amyloglucosidase from A. niger was covalently immobilized onto poly (GMA-co-EGDMA) by the glutaraldehyde and periodate method. The immobilization of amyloglucosidase after periodate oxidation gave a preparate with the highest specific activity reported so far on similar polymers. The obtained immobilized preparates show the same pH optimum, but a higher temperature optimum compared with the soluble enzyme. The kinetic parameters for the hydrolysis of soluble starch by free and both immobilized enzymes were determined.
%K glucoamylase
%K poly(GMA-co-EGDMA)
%K immobilization
%K periodate
%K starch
%U http://www.shd.org.yu/HtDocs/SHD/vol70/No5/JSCS_V70_No5-05.pdf