%0 Journal Article
%T Boron isotope dilution in cellular fractions of coffee leaves Evaluated by Inductively Coupled Plasma Mass Spectrometry with direct injection nebulization (DIN-ICP-MS)
%A Bellato Ana Cl¨¢udia S.
%A Meneg¨¢rio Amauri A.
%A Gin¨¦ Maria Fernanda
%J Journal of the Brazilian Chemical Society
%D 2003
%I Sociedade Brasileira de Qu¨ªmica, SBQ
%X Enriched 10B (94.14 atom %) was supplied to coffee plantlets for three months. Then boron isotope ratios were determined in the leaf cell compartments, cell wall, nuclei and chloroplast, after a sub-cellular fractionation procedure. The isotopic measurements were performed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) provided with a direct injection nebulizer (DIN), introducing a sample volume of 50 muL. Isotopic ratios from 1.002 to 1.326 were determined with precision characterized by RSD lower than 1.5% for the enriched cell fractions with B concentrations ranging from 3.3 to 10.8 mug g-1. The detection limit (3sigma) was 0.5 ng B mL-1. The average enrichments in 10B atom % found in the cell walls, nuclei and chloroplasts were 46.7, 44.5 and 48.8, respectively.
%K isotope dilution
%K inductively coupled plasma mass spectrometry
%K biological cell fractionation
%K boron isotope ratios
%K plant analysis
%U http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532003000200014